Circulating type phytoplankton species and cell density detection device and method

Abstract

The invention discloses a circulating type phytoplankton species and cell density detection device. The device comprises a shell, a data acquisition and processing module and an upper computer module;a flow chamber, a fluorescence detection module and a holographic imaging detection module are arranged in the shell; the fluorescence detection module comprises an excitation light source illumination light path and a fluorescence detection light path which are arranged orthogonally; the fluorescence detection module and the holographic imaging detection module are respectively connected with adata acquisition and processing module; and the data acquisition and processing module is connected with the upper computer module. According to the invention, a circulating structure is adopted to continuously detect phytoplankton species and cell concentration in seawater; a multi-channel fluorescence detection light path is constructed, and the collection efficiency of weak light signals is improved; the holographic imaging detection light path is designed to overcome the limitation of small depth of field of traditional microscopic imaging; the device has the advantages of low cost, compact structure, small size, no influence of natural environment illumination and the like, and can realize automatic identification of phytoplankton species in water and rapid measurement of cell concentration.

Description

Technical field [0001] The invention relates to the field of environmental sciences, in particular to a circulation type phytoplankton species and cell density detection device and detection method. Background technique [0002] Phytoplankton is the most important producer and energy converter in the marine ecosystem. It forms the nutritional foundation of the marine food web. As a photosynthetic organism, it is also a major contributor to the global carbon cycle. Under certain conditions, phytoplankton explosively accumulate or multiply in seawater, which causes the water body to change color or cause damage to other marine life. Generally, the density of algae cells is 10 6 -10 7 When cell / L, it is considered that red tide has broken out. In recent years, the frequency of red tides in the coastal areas of my country has continued to increase, and the affected areas have continued to expand, posing a serious threat to the sustainable development of aquaculture in coastal areas, ...

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Problems solved by technology

[0005] The invention aims to overcome the problems of complex structure, complicated operation and high cost of the ph

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