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Biosynthetic compound with high light resistance
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A biosynthesis and compound technology, applied in the fields of luminescent materials, organic chemistry, chemical instruments and methods, etc., can solve the problems of unstable chromophore group, poor light fastness, affecting the dyeing effect of dyes, etc. The effect of easy availability of raw materials and easy industrialization
Pending Publication Date: 2020-09-04
杭州唯铂莱生物科技有限公司
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Problems solved by technology
Although in the dyeing process of natural dyes, the use of mordant dyeing and some post-treatment can improve the color fastness, but due to the inherent instability of the chromophoric group of natural dyes, the color fastness
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Embodiment 1
[0021] Preparation of engineering bacteria for dye preparation:
[0022] With reference to CN102952813B (synthesis method and extraction process of blue natural dye), engineering bacteria for biosynthesis and transformation are prepared.
Embodiment 2
[0024] Preparation of Compound A:
[0025]
[0026] Will 5g Add it into the engineering bacteria culture solution in Example 1, after 24 hours of cultivation, collect the culture solution, obtain the precipitated substance after centrifugation, use DMSO to dissolve the precipitate, and remove the insoluble matter, and evaporate the DMSO to dryness to obtain ash Brown solid powder, yield 92%.
Embodiment 3
[0028] Preparation of Compound B:
[0029]
[0030]
[0031] Will 5g Add it to the engineering bacteria culture solution in Example 1, after 28 hours of cultivation, collect the culture solution, obtain the precipitated substance after centrifugation, dissolve the precipitate with DMSO, and remove the insoluble matter, and evaporate and dry the DMSO to obtain dark blue Color solid powder, yield 95%.
[0032] Example 3
[0033] Preparation of Compounds C, D, E:
[0034]
[0035] 3g and 3g After 1:1 mixing, it was added to the engineering bacterium culture solution in Example 1. After 32 hours of cultivation, the culture solution was collected, and the precipitate was obtained after centrifugation. The precipitate was dissolved with DMSO, and the insoluble matter was removed. Phase treatment, collect the effluent at 12-15 minutes, 20-25 minutes, and 30-33 minutes respectively, and vacuum dry to obtain dark brown solid powder (C), yellow-brown solid powder (D), a...
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Abstract
The invention discloses a biosynthetic compound with high light resistance. The biosynthetic compound has a molecular structural general formula, wherein R1, R2, R3 and R4 are H,-OH,-NO2,-CN,-NH2,-CH3or halogen; the compound with the structural formula can be used as a dyeing dye and a fluorescent probe. When the compound is used as a dye, the compound has the beneficial effects that (1) the compound has the characteristics of a reduced dye, can be dissolved in water to be prepared in a certain reaction mode, and can be oxidized and restored to present a specific color through an oxidation means; (2) according to ISO 105-B02: 2013 artificial light color fastness to light: xenon arc lamp test, the color fastness to light can be far higher than that of the traditional natural dye, and the color fastness to light is superior to the 5-grade or higher color fastness to light of part of artificially and chemically synthesized dyes at the same time; and (3) safety, no toxicity, readily available raw materials and simple structure are realized, large-scale production can be realized in a combined biological manner, and industrialization is facilitated.
Description
technical field [0001] The invention belongs to fine chemical high-tech functional dyes, in particular to a biosynthesized compound with high light fastness. Background technique [0002] Natural dyes refer to dyes obtained from plants, animals, microorganisms or mineral resources without artificial chemical synthesis or processing. Natural dyes are soft in color, mostly non-toxic and harmless, non-allergic and carcinogenic to the skin, and have good biodegradability and environmental compatibility. In addition to the dyeing function, they also have various functions such as medicine and fragrance. [0003] The dye is excited after absorbing photons, and the structure is destroyed through a series of photochemical reactions, which is prone to discoloration or fading. Therefore, the chemical structure, aggregation state, and combination state of the dye determine the color fastness to light of the textile. Although in the dyeing process of natural dyes, the use of mordant d...
Claims
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