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Micromonas haikou with algae-dissolving ability and its application to Karenia mikarii

A kind of technology of Karenella mikielii and Micromonospora, applied in the field of environmental microorganisms

Active Publication Date: 2022-05-10
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports of algae-dissolving bacteria and bacterial agents that are highly effective in killing Karenia militaris

Method used

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  • Micromonas haikou with algae-dissolving ability and its application to Karenia mikarii
  • Micromonas haikou with algae-dissolving ability and its application to Karenia mikarii
  • Micromonas haikou with algae-dissolving ability and its application to Karenia mikarii

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Experimental program
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Embodiment

[0030] 1. Screening of algae-lytic bacteria

[0031] Collect the soil attached to the surface of the roots of Sangria apetalus (within 5mm from the roots), weigh 2.0g of soil samples and put them in a conical flask with 20ml of sterile water (with glass beads inside), shake them up by hand; 10 -2 and 10 -3 The diluted samples were inoculated in AGG solid medium and cultured at 28°C for 3 days. Each grown single colony was inoculated in the improved ISP2 liquid culture medium, and cultured at 28°C and 200rpm for 3 days. Inoculate 0.1 mL of bacterial liquid into 5 mL of aseptically treated algae liquid of Karenia militaris in logarithmic growth phase, culture at 20°C for 2, 4, 6, 12, 24 and 48 hours, take 1 mL of algal liquid each, and use water Count the algal cells on the sample counting plate, and the strains that cause a large number of algal cell death are considered as alginolytic bacteria.

[0032] A bacterium with the best algalytic effect was selected for further re...

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Abstract

The invention discloses a Micromonas haikou strain with algae-dissolving ability and its application in killing Karenia mikimotoi, belonging to the field of microbiology of harmful red tide treatment. The algalytic bacterium is named Micromonospora haikouensis B2572 (hereinafter referred to as Micromonas haikou B2572 or strain B2572), which was isolated from the rhizosphere of Sangria apetalum in Maoweihai Mangrove Nature Reserve, Qinzhou City, Guangxi. It was deposited in China Center for Type Culture Collection on March 22, deposit number: CCTCC NO: M 2019382. The new bacterial strain provided by the present invention has a very good inhibitory and killing effect on the dominant algal species Karenia militaris in the Beibu Gulf sea area, and the algae-dissolving rate reaches more than 90% under 24h treatment conditions, and the harmful algal blooms that break out in large-area water bodies Event application prospects are broader.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to Micromonas haikou with algae-dissolving ability and its application to Karenia militaris. Background technique [0002] Karenia mikari is a species widely distributed in the world. It can secrete hemolytic toxins and cytotoxins to harm fish and marine invertebrates. It has become the dominant species of red tide algae in my country. According to data, the red tide of Karenia militaris presents a growing trend, and it lasts for a long time and affects a wide range. In addition, Karenia mikari often forms biphasic red tides with Prorocentrum donghaiense, which seriously damages the marine ecosystem. Therefore, it is particularly urgent and necessary to carry out research on the prevention and control of K. mikari algae red tide. [0003] For the control of red tide, there are mainly physical, chemical and biological methods. Although physical met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC12N1/20C02F3/34C12R2001/01C12N1/205
Inventor 李菲覃仙玲牙韩争陈波潘信利李喆姜发军
Owner GUANGXI ACAD OF SCI
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