An integrated self-service nucleic acid detection device and its use method

Abstract

The invention relates to an integrated self-service nucleic acid detection device and its use method. The device comprises: a cartridge, on which a piston chamber, a sample receiving chamber, a reagent chamber and a recovery chamber are formed; a reaction chip, on which Two reaction pools are formed on the surface; the elastic membrane bonds the reaction chip to the bottom of the cartridge; the sample receiving chamber and the reagent chamber communicate with a reaction pool through the first one-way valve structure, and the reagent chamber and the recovery chamber It communicates with another reaction pool through the second one-way valve structure. The present invention only needs 4 simple steps of pushing and pulling the push rod to complete the whole process of nucleic acid detection, not only does not require professional training and special laboratory conditions, but also does not need equipment such as pipetting guns and temperature cycle instruments, which greatly simplifies the operation of nucleic acid detection The steps reduce the requirements for operators and operating places, and can be widely used in families and community clinics to realize real-time screening and detection during epidemic prevention and control, as well as personal daily health monitoring.

Description

technical field [0001] The present invention relates to a nucleic acid detection device and its use method, in particular to an integrated self-service nucleic acid detection device and its use method that can simply and conveniently realize nucleic acid amplification detection at home or in any other place. Background technique [0002] Nucleic acid detection is the most direct, reliable and sensitive method in pathogen detection, and has important applications in disease diagnosis, epidemic prevention and control, health monitoring and other fields. Nucleic acid detection detects the genetic material (DNA or RNA) of the pathogen, and can determine the type and concentration of the pathogen within 1 hour, avoiding the interference of factors such as the window period of disease infection. [0003] The nucleic acid detection process generally includes the following steps: sample pretreatment, nucleic acid extraction, nucleic acid amplification, and detection of amplification...

AI Technical Summary

Problems solved by technology

Therefore, the high threshold of traditional nucleic acid detection technology limits the huge application prospect of this technology

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