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Preparation method and application of cell membrane coated nano topological structure array

A topological structure and cell membrane technology, which is applied in the modification of cell membranes, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of complicated and time-consuming operations, difficult preparations, limited application areas, etc., and achieve efficient capture, preparation and Simple operation effect

Active Publication Date: 2020-09-11
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For this reason, the technical problem to be solved by the present invention is to overcome the problems of difficult preparation, complex and time-consuming operation and limited application in the prior art, thereby providing a preparation and Preparation method and application of cell membrane-coated nano-topological structure array with simple operation and limited application

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  • Preparation method and application of cell membrane coated nano topological structure array
  • Preparation method and application of cell membrane coated nano topological structure array
  • Preparation method and application of cell membrane coated nano topological structure array

Examples

Experimental program
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Embodiment 1

[0027] Such as figure 1 As shown, this embodiment provides a method for preparing a cell membrane-coated nano-topological structure array, including the following steps: Step S1: Stimulate macrophages 10 to form stimulated macrophages 11, and extract stimulated macrophage membranes 12; at the same time, the substrate 20 is processed to form a substrate 21 with nanowires, such as Figure 2a As shown, the substrate 21 with nanowires is processed to form a positively charged nanowire substrate 22, such as image 3 shown; step S2: combining the stimulated macrophage cell membrane 12 with the positively charged nanowire substrate 22, as Figure 2b As shown, a macrophage membrane-modified nanotopographic structure array 30 is obtained.

[0028] The preparation method of the cell membrane-coated nano-topological structure array described in this embodiment, in the step S1, the macrophage 10 is stimulated to form a stimulated macrophage 11, and the stimulated macrophage membrane 12 ...

Embodiment 2

[0051] Such as figure 1 As shown, this embodiment provides the application of the first cell membrane-coated nano-topographic structure array. According to the preparation method of the cell membrane-coated nano-topological structure array described in Example 1, a macrophage membrane-modified nano-topological structure array 30 is obtained. The macrophage membrane-modified nano-topographic structure array 30 is used as a substrate, and is used as a medical dressing to buckle on a culture plate containing colonies.

[0052] This embodiment provides the application of the first cell membrane-coated nano-topological structure array. According to the preparation method of the cell membrane-coated nano-topological structure array described in Example 1, a macrophage membrane-modified nano-topological structure array 30 is obtained. The macrophage membrane-modified nano-topological structure array 30 is used as a substrate, and is used as a medical dressing to cover a culture plate...

Embodiment 3

[0055] Such as figure 1 and image 3 As shown, this embodiment provides the application of the second cell membrane-coated nano-topological structure array, including: manufacturing a microfluidic chip, setting a chip channel in the microfluidic chip; combining the microfluidic chip with the embodiment A method for preparing the cell membrane-encapsulated nano-topographic structure array is combined with 30 macrophage membrane-modified nano-topological structure arrays, such as Figure 4 Shown; Liquid samples are injected into the chip channel to specifically capture and remove bacteria.

[0056] This embodiment provides the application of the second cell membrane-coated nano-topological structure array, including: manufacturing a microfluidic chip, and setting a chip channel in the microfluidic chip, which is conducive to injecting liquid samples into the chip channel; The microfluidic chip is combined with the macrophage membrane-modified nano-topographic structure array 3...

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Abstract

The invention relates to a preparation method and an application of a cell membrane coated nano topological structure array. The preparation method comprises the following steps: stimulating macrophages to form stimulated macrophages, and extracting stimulated macrophage membranes; meanwhile, processing the substrate to form a substrate with nanowires, and processing the substrate with the nanowires to form a nanowire substrate with positive charges; and combining the stimulated macrophage membrane with the nanowire substrate with the positive charges to obtain the macrophage membrane modifiednano topological structure array. The cell membrane coated nano topological structure array is simple in preparation and operation, and can be applied to capture of bacteria.

Description

technical field [0001] The invention relates to the technical field of cell membrane-encapsulated nanoparticles, in particular to a preparation method and application of a cell membrane-encapsulated nano-topological structure array. Background technique [0002] Chemically modify functional biological proteins on the surface of the topological structure of the material to prepare a platform for capturing bacteria and monitoring them efficiently. Such as Xiaodong Chen et al. (Yong-Qiang Li, Bowen Zhu, Yuangang Li, Wan Ru Leow, Rubayn Goh, Bing Ma, Eileen Fong, Mark Tang, and Xiaodong Chen; A Synergistic Capture Strategy for Enhanced Detection and Elimination of Bacteria.Angewandte Chemie.2014 , 5947) modified lysozyme or concanavalin A to the surface of silicon nanowire arrays to efficiently capture and kill bacteria. [0003] Although the above can efficiently capture and kill bacteria, it uses chemical bonding to modify the protein on the surface of the material, which req...

Claims

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Application Information

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IPC IPC(8): C40B50/06C12N5/0786C12N1/20C12R1/19C12R1/445
CPCC40B50/06C12N5/0645C12N1/20A61K35/15C12N2537/00C12N5/0081C12N2502/70A61L2400/12A61L15/46A61L15/26A61L15/42A61L2300/30A61P31/00C08L83/04A61L15/40A61L2420/02C12N5/0006C12N11/14
Inventor 刘坚刘思迪肖翔
Owner SUZHOU UNIV
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