LncRNA marker related to porcine muscle fiber types and application of lncRNA marker

A technology for muscle fiber types and markers, which is applied to the field of molecular marker preparation in livestock, can solve problems such as reduced pork quality

Active Publication Date: 2020-09-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, my country's commercial pigs are mainly lean-meat pig breeds imported from abroad, with fast growth, high lean meat rate and high feed conversion rate, but the quality of pork is significantly reduced

Method used

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  • LncRNA marker related to porcine muscle fiber types and application of lncRNA marker
  • LncRNA marker related to porcine muscle fiber types and application of lncRNA marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Different breeds of pig muscle fiber types and high-throughput lncRNA-seq sequencing analysis

[0039] 1) Select Landrace pigs with large differences in carcass muscle fiber types and Guangdong special breed Lantang pigs as research objects; select 3 cases of one-day-old Landrace pigs and three cases of one-day-old Blue Tang pigs to take their back muscle tissue and tissue samples respectively After taking it out, it was quickly frozen in liquid nitrogen for 30 seconds, and then stored in a -80°C refrigerator. Then a part was subjected to paraffin-embedded section and muscle fiber hematoxylin-eosin staining analysis, and the other part was sent to the sequencing company with dry ice for lncRNA sequencing.

[0040] 2) There are obvious differences in muscle fiber appearance in the back muscle tissue of one-day-old lean-meat Landrace pigs and fat-type Blue Tang pigs. The longitudinal sectional area was lower than that of Landrace pigs, and the cross section of a...

Embodiment 2

[0046] Example 2 Fluorescence quantitative PCR detects the expression of LincRNA-ROMT in pig muscle tissue

[0047] Reagent:

[0048] Reverse transcription kit: PrimeScript TM RT reagent Kit with gDNA Eraser (Perfect RealTime), #RR047Q, Takara;

[0049] Quantitative PCR reagents: Fast qPCR Mix, #RR430A, Takara.

[0050] Relevant experimental items to RNase treatment:

[0051] 1) Rinse and soak all glassware with DEPC before use, high pressure at 120°C for 20 minutes, and dry at high temperature at 180°C for more than 2 hours.

[0052] 2) Soak plastic utensils (such as EP tubes / tips) in 0.1% DEPC water overnight before use, and then dry the liquid, 120°C and high pressure for 20 minutes, and dry in an oven for later use.

[0053] Total RNA extraction:

[0054] Select 50 cases of Landrace pig muscle tissue and 50 cases of Lantang pig muscle tissue, and use the TRIzol method according to the instructions of the Invitrogen kit. The specific steps are as follows:

[0055] 1...

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Abstract

The invention discloses an lncRNA marker related to porcine muscle fiber types and an application of the lncRNA marker, and belongs to the technical field of livestock molecular marker preparation. The lncRNA marker is LincRNA-ROMT, and the sequence of the lncRNA marker is as shown in SEQ ID NO:1. The LincRNA-ROMT is closely related to the porcine muscle fiber types, and extremely remarkably showslow expression in oxidized porcine muscle fibers (fat type Lantang porcine muscle tissues). The lncRNA marker can be applied to an auxiliary genetic breeding process in the aspect of pig variety improvement. The LincRNA-ROMT disclosed by the invention can be used as a molecular marker related to porcine muscle fiber types, and has important practical application values in genetic assisted breeding.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker preparation, and in particular relates to an lncRNA marker related to pig muscle fiber type and application thereof. Background technique [0002] Muscle formation and quality trait improvement of livestock and poultry is an important research direction of animal genetics and breeding. At present, my country's commercial pigs are mainly lean-meat pig breeds imported from abroad, with fast growth, high lean meat rate and high feed conversion rate, but the quality of pork is significantly reduced. In recent years, with the increasing demand of consumers for high-quality meat products, there is an urgent need to cultivate high-quality meat pigs and improve meat quality. Muscle fiber is the basic unit of muscle tissue, and the type of muscle fiber is closely related to the quality of muscle, especially the tenderness of food quality. Therefore, at the level of pig ketone body musc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/158C12Q2600/124C12Q2600/178C12Q2563/107C12Q2545/101C12Q2531/113
Inventor 孙加节张永亮江青艳习欠云陈婷罗君谊
Owner SOUTH CHINA AGRI UNIV
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