Live embryo surface zona pellucida tension detection device

A tension detection, transparent belt technology, applied in the field of medical devices, can solve the problems of unsatisfactory transparent belt, high temperature, bad chemical change and so on

Pending Publication Date: 2020-09-22
梁诗豪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The usual test-tube baby is called assisted reproductive technology in the industry. A traditional assisted reproductive technology includes in vitro fertilization. When the patient's eggs are surgically removed after ovulation induction, the eggs are combined with the husband's sperm outside the body. After 3-6 days of culture, the embryos You can choose to be transplanted back to the mother to complete the assisted conception process, but the research data shows that if the patient is older than 35 years old, the tension of the zona pellucida on the surface of the patient's embryo will become too large (commonly known as the zona pellucida is too hard), making it difficult for the embryo to hatch in the mother's body. Assisted hatching is required, an

Method used

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  • Live embryo surface zona pellucida tension detection device

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Such as figure 1 As shown, a device for detecting the tension of zona pellucida on the surface of living embryos includes an inverted microscope 1. The inverted microscope 1 uses a Nikon inverted microscope of Ti-E / Ti-U / Ti-S, and the inverted microscope 1 is equipped with an Eppendorf microscope. Operating kit, the inverted microscope 1 includes a base 2, a mirror arm 3 fixedly connected to the upper surface of the base 2, a condenser 4 fixedly connected to the top of the mirror arm 3, an objective lens 5 located on the upper surface of the base 2, and a first The support arm 8 is connected to the first operating arm 9 of the mirror arm 3, the second operating arm 16 is connected to the mirror arm 3 through the second support arm 19, and the first operating arm 9 is connected to the first rocker 12 through the first signal line 13A , By operating the first rocker 12, the first operating arm 9 can be moved in the horizontal and vertical directions, the second operating arm...

Embodiment 2

[0033] Such as figure 1 As shown, a device for detecting the tension of zona pellucida on the surface of living embryos includes an inverted microscope 1. The inverted microscope 1 uses a Nikon inverted microscope of Ti-E / Ti-U / Ti-S, and the inverted microscope 1 is equipped with an Eppendorf microscope. Operating kit, the inverted microscope 1 includes a base 2, a mirror arm 3 fixedly connected to the upper surface of the base 2, a condenser 4 fixedly connected to the top of the mirror arm 3, an objective lens 5 located on the upper surface of the base 2, and a first The support arm 8 is connected to the first operating arm 9 of the mirror arm 3, the second operating arm 16 is connected to the mirror arm 3 through the second support arm 19, and the first operating arm 9 is connected to the first rocker 12 through the first signal line 13A , By operating the first rocker 12, the first operating arm 9 can be moved in the horizontal and vertical directions, the second operating arm...

Embodiment 3

[0041] Such as Picture 9 with Picture 10 As shown, the simulation experiment is as follows: the embryo model made by the flat extrusion balloon can be used to verify the feasibility of the instrument measurement, and the actual extrusion model is equivalent to Picture 9 The embryo tension measurement process shown in the whole process is that two standard planes are squeezed in parallel to measure the tension of the sphere. The ring is a petri dish. The scale in the figure is the stopper 20 in the petri dish, with the measured length on it. The scale used, the compressed pie-shaped object is the embryo model in the petri dish. When the embryo model is not compressed, the compressed distance is 0. Each time you change the compression distance of the embryo extrusion needle, record it in the table below, and Convert it to m as the unit to express, record the sensor reading (refitted from an electronic balance), convert the sensor reading to kg and multiply it by 9.8N / kg to get ...

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Abstract

The invention discloses a live embryo surface zona pellucida tension detection device. The device comprises an inverted microscope, further comprises a pressure sensor which is fixedly connected witha first operating arm of the inverted microscope, an extrusion needle which is fixedly connected with the other end of the pressure sensor and is used for extruding an embryo, a fixed needle which isfixedly connected with a second operating arm of the inverted microscope, and a micromanipulation vessel which is positioned on an objective table of the inverted microscope, wherein the pressure sensor is electrically connected with the computer through a data line, and the pressure sensor transmits detected data to the computer for storage. The device is advantaged in that the pressure sensor isadditionally arranged on an existing inverted microscope, a camera on the inverted microscope is combined, collection and research of tension-related data of a transparent belt on a surface of a tested living embryo are facilitated, a separation blade is arranged in the micromanipulation vessel so that a supporting surface is provided for the living embryo conveniently, a zona pellucida on the surface of the living embryo is slightly deformed under the combined action of the extrusion needle, the fixed needle and the separation blade, the living embryo is not damaged, finally, a tension coefficient of the living embryo is calculated, and embryologists can judge activity of the living embryo by using data and common indexes for embryo judgment conveniently.

Description

Technical field [0001] The invention relates to the technical field of medical equipment, in particular to a zona pellucida tension detection device on the surface of a living embryo. Background technique [0002] The usual test-tube baby is called assisted reproductive technology in the industry. A traditional assisted reproductive technology includes in vitro fertilization. When the patient’s egg is surgically removed after ovulation induction, the egg is combined with the husband’s sperm outside the body. After 3-6 days of culture, the embryo You can choose to be transplanted back to the mother to complete the assisted conception process, but the research data shows that if the patient is more than 35 years old, the zona pellucida on the surface of the patient's embryo will become too strong (commonly known as the zona pellucida), making it difficult for the embryo to hatch in the mother. It requires assisted incubation. The laser method is now more popular. Install the laser ...

Claims

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Application Information

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IPC IPC(8): G01L5/04
CPCG01L5/04
Inventor 梁诗豪葛英辉张翠莲谢娟珂
Owner 梁诗豪
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