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Improved specific growth factor detection kit and application thereof

A technology for detecting kits and growth factors, which is applied in the field of biochemistry, can solve the problems of limited types of detection products and the need to improve the measurement accuracy, and achieve the effects of high detection rate, rapid response and good stability

Active Publication Date: 2020-09-29
HUNAN NEWLAND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the types of TSGF detection products on the market are limited, and the measurement accuracy needs to be improved

Method used

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  • Improved specific growth factor detection kit and application thereof
  • Improved specific growth factor detection kit and application thereof
  • Improved specific growth factor detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This kit adopts visible light spectrophotometry, under the detection system of 37 ℃, the abnormally produced tumor-specific growth factors in the sample, such as bFGF, TGF-β, HSGF, specifically combine with heparan sulfate to form complex A. The formation of substance A reduces the concentration of complex B formed by the combination of heparan sulfate and chromogen, resulting in a sharp drop in the absorbance value of the system. The concentration of complex B is quantitatively detected by converting the standard curve and the formula, thus indirectly reflecting the presence of TSGF in the sample. in the level.

[0020] 1. Reagent preparation

[0021] (1) Preparation of R1 reagent

[0022] Weigh the following ingredients:

[0023]

[0024] Dissolve it in purified water and prepare a 100mL solution, which is the R1 reagent.

[0025] (2) Preparation of R2 reagent

[0026] Weigh the following ingredients:

[0027]

[0028] Dissolve it in purified water and prep...

Embodiment 2

[0102] This kit adopts the immunoturbidimetric method. Under the detection system of 37°C, the tumor-specific growth factor in the sample undergoes an agglutination reaction with the latex particles coupled with the corresponding monoclonal antibody to form an antigen-antibody complex. (A wavelength within the range of 550nm to 580nm can also be selected) to measure the absorbance value of the complex, and convert the production amount of the quantitative detection product through the standard curve and formula conversion, which can indirectly reflect the level of TSGF in the sample to be tested.

[0103] 1. Reagent preparation

[0104] (1) Preparation of R1 reagent

[0105] Weigh the following ingredients:

[0106]

[0107]

[0108] Dissolve it in purified water and prepare a 100mL solution, which is the R1 reagent.

[0109] (2) Preparation of R2 reagent

[0110] Weigh the following ingredients:

[0111]

[0112] Dissolve it in purified water and prepare a 100mL ...

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Abstract

The invention discloses an improved specific growth factor detection kit, and belongs to the technical field of biochemistry. The kit comprises a reagent R1, a reagent R2 and a calibrator; the reagentR1 is mainly a buffer solution component, and the reagent R2 is mainly a reaction solution. The kit provided by the invention has high sensitivity and specificity, has early stage and broad spectrumfor detection of tumor samples, and can be suitable for early stage screening of various tumors.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to an improved specific growth factor detection kit and its application in tumor sample detection. Background technique [0002] Specific growth factor (TSGF) is a class of independent substances different from other tumor markers. It is a general term for a series of factors that promote the proliferation of tumors and their surrounding capillaries and release them into peripheral blood during the formation and growth of malignant tumors. These factors are produced by the tumor in an autocrine and paracrine manner, mainly composed of carbohydrates (such as glycolipids, glycoproteins, oligosaccharides, etc.), including epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), tumor growth factor α and β (TGF-α, TGF-β), human bone growth factor (HSGF), and angiopoietin (ANG ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/577G01N33/543G01N21/78G01N21/31
CPCG01N33/57415G01N33/57423G01N33/57488G01N33/577G01N33/5432G01N21/78G01N21/31G01N2333/503G01N2333/495G01N2333/65Y02A50/30
Inventor 汪媛媛薛原楷
Owner HUNAN NEWLAND BIOTECH