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A kind of in vitro bud cultivation method of panicle hydrangea

A cultivation method and technology of conical hydrangea, which are applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve problems such as no relevant reports, and achieve the effects of high reproduction rate, low browning rate and low pollution rate.

Active Publication Date: 2022-03-11
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no relevant report on the research on the in vitro bud culture technology of Pane hydrangea 'Pink Fairy'

Method used

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  • A kind of in vitro bud cultivation method of panicle hydrangea
  • A kind of in vitro bud cultivation method of panicle hydrangea
  • A kind of in vitro bud cultivation method of panicle hydrangea

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Experimental program
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Embodiment Construction

[0025] 1. Material: 'Pink Fairy', one of the panicle hydrangea varieties, select the terminal buds and axillary buds of the young and tender stems of the year.

[0026] 2. Methods and steps:

[0027] 2.1 Collection of explants: The time is selected from April to June, under the conditions of fine weather and suitable humidity, and the selected variety is 'Pink Fairy'. Select spring shoots that grow vigorously, are free from diseases and insect pests, and have full axillary buds as materials. When collecting explants, it is best to take the young and tender internal branches of the year, so as to avoid unnecessary losses to the plants. Immediately after collection, put the cut part into a container filled with water to prevent dehydration and inactivation, and then process and inoculate in time.

[0028] 2.2 Material pretreatment: Trim the collected 'Pink Fairy' branches, remove the leaves and leave some petioles, cut them into small sections of about 2cm, and keep at least 1...

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Abstract

The invention discloses a method for in vitro bud cultivation of Hydrangea paniculata. Rinse with sterile water, and then carry out surface disinfection: soak in 75% alcohol, rinse with sterile water, HgCl 2 Soak in the solution and rinse with sterile water; trim the incisions at both ends of the sterilized explants, cut them into stems with buds, and inoculate them on the starting medium in an upright manner; cut the tissue cultured seedlings that have been cultured For the stem section with buds, remove the leaves that can touch the medium, and inoculate vertically in the proliferation medium; after the proliferation culture, trim the base of the tissue cultured seedlings, and transfer them to the rooting medium for root induction. The method has good starting effect, low browning rate and pollution rate, the browning rate is 0, and the pollution rate is as low as 5%; the germination induction rate and the multiplication coefficient are high, the germination rate reaches 100%, and the multiplication coefficient reaches 7; the rooted seedlings are strong and The rooting rate reaches 100%, the average root quantity reaches 22, and the average root length is 3.10cm.

Description

technical field [0001] The invention relates to plant cultivation technology, in particular to a method for cultivating isolated buds of hydrangea paniculata. Background technique [0002] Hydrangea paniculata Sieb.et Zucc is a deciduous shrub belonging to the genus Hydrangea in the saxifrage family. Branches dark reddish brown or taupe. Leaves are papery, 2-3 opposite or in whorls, ovate or elliptic, with serrated margins and hairy backs. Pane hydrangea is large and beautiful, with peculiar flower shape, up to 30-40cm long, flowering period from July to October, long flowering period and pleasant color. Native to Japan and Sichuan, China. It has been widely used and studied since it was introduced to Europe in the 18th century. It is a high-end flower, but it has not been popularized in China. It can be planted in the courtyard to make flower fences and flower mirrors, or cut off the flower bulbs and put them in vases indoors, which has high ornamental value. Not only ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈婷婷孟紫莲王红梅刘春风胡众恒
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY