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Method for increasing squalene content in siraitia grosvenori

A technology of Luo Han Guo and squid, which is applied in the field of plant biology, can solve the problems of low content and restrict the healthy development of the Luo Han Guo industry, and achieve the effects of promoting accumulation, improving disease resistance and survival rate, and promoting plant root development

Inactive Publication Date: 2017-04-26
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that squalene is an intermediate product in the biosynthesis of glucoside V. In the late development of Luo Han Guo fruit, squalene is completely consumed and converted into glucoside V. However, squalene only exists in less than 15% of the fruit weight. In the pulp, and the content is extremely low, which seriously restricts the healthy development of the Luo Han Guo industry

Method used

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  • Method for increasing squalene content in siraitia grosvenori

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Experimental program
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Effect test

Embodiment 1

[0033] S1. Tissue culture the explants of Luo Han Guo in a solid medium containing methyl jasmonate to obtain Luo Han Guo seedlings;

[0034] S2. Cultivate Luo Han Guo seedlings, during which 50 μmol / L methyl jasmonate solution is sprayed on the surface of Luo Han Guo seedlings 10 days after pollination until the surface drips.

Embodiment 2

[0036] S1. Tissue culture the explants of Luo Han Guo in a solid medium containing methyl jasmonate to obtain Luo Han Guo seedlings;

[0037] S2. Cultivate Luo Han Guo seedlings, during which 400 μmol / L methyl jasmonate solution is sprayed on the surface of Luo Han Guo seedlings 20 days after pollination until the surface drips.

Embodiment 3

[0039] S1. Tissue culture the explants of Luo Han Guo in a solid medium containing methyl jasmonate to obtain Luo Han Guo seedlings;

[0040] S2. Cultivate Luo Han Guo seedlings, during which 200 μmol / L methyl jasmonate solution is sprayed on the surface of Luo Han Guo seedlings 15 days after pollination until the surface drips.

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Abstract

The invention discloses a method for increasing the squalene content in siraitia grosvenorii. The method comprises the following steps that S1, tissue culture of siraitia grosvenorii explants is conducted in a solid culture medium containing methyl jasmonate to obtain siraitia grosvenorii seedlings; and S2, the siraitia grosvenorii seedlings are cultivated, during cultivation, the surface of the siraitia grosvenorii seedlings which are pollinated for 10-20 d is sprayed by 50-400 [mu]mol / L of methyl jasmonate solutions until water dripping is formed on the surface. The methyl jasmonate are added in the process of siraitia grosvenorii tissue culture and cultivation, and high expression of a metabolic key enzyme gene of siraitia grosvenorii squalene is induced, so that the content of the siraitia grosvenorii squalene is quickly increased in a short time.

Description

technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for increasing the content of squalene in Luo Han Guo. Background technique [0002] Luo Han Guo (Siraitia grosvenorii) is a precious medicinal and sweet plant unique to China. Its fruit is cool in nature and sweet in taste, and has the functions of clearing away heat and moistening lungs, relieving throat and opening sound, laxative and anticancer. The active ingredient glucoside V is one of the strongest non-sugar sweeteners in the world. It is 300-400 times higher than sugar, widely used in food, health products and medicines, and is an ideal sugar substitute for diabetics, obese people and hypertensive patients. Studies have found that squalene is an intermediate product in the biosynthesis of glucoside V. In the late development of Luo Han Guo fruit, squalene is completely consumed and converted into glucoside V. However, squal...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00C05G3/00
CPCA01G22/00A01H4/00A01H4/001A01H4/008C05D9/00C05G3/00C05F11/00
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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