Application of GSDMD as early diagnosis marker of myocardial ischemia-reperfusion injury
A technology for reperfusion injury and myocardial ischemia, which is applied in the field of biomedicine and can solve the problems of lack, no related reports on expression and effect, etc.
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[0033] To establish a mouse model of ischemia-reperfusion injury and detect the level of GSDMD, the specific operations are as follows:
[0034] (1) Establishment of the mouse model of ischemia-reperfusion injury: After the mouse was anesthetized with 2% isoflurane, the chest area was depilated, sterilized with 75% alcohol, spread a towel, and a small Xmm opening was made at the X rib, and blunt dissection Muscle, stretch the ribs and extrude the heart, ligate the coronary artery at Xmm below the left atrial appendage with 6-0 silk thread, tie a slipknot, suture the incision with 4-0 silk thread, check the heart for ischemia by electrocardiogram, loosen the slipknot after 40 minutes, and observe the electrocardiogram Whether to reperfuse.
[0035] (2) Detection of GSDMD level, specifically:
[0036] Western blot: The mouse heart samples were removed and frozen in liquid nitrogen, and transferred to -80°C for storage after 24 hours. After the tissue was shredded, RIPA lysate ...
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