Application of GSDMD as early diagnosis marker of myocardial ischemia-reperfusion injury

A technology for reperfusion injury and myocardial ischemia, which is applied in the field of biomedicine and can solve the problems of lack, no related reports on expression and effect, etc.

Pending Publication Date: 2020-10-02
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on GSDMD and pyroptosis mainly focuses on immune-related macrophages and neutrophils, etc. There is no related report on the expression and role of GSDMD in cardiomyocytes, and the role of GSDMD in cardiac ischemia-reperfusion is also unknown. There is a lack of relevant studies confirming

Method used

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  • Application of GSDMD as early diagnosis marker of myocardial ischemia-reperfusion injury
  • Application of GSDMD as early diagnosis marker of myocardial ischemia-reperfusion injury
  • Application of GSDMD as early diagnosis marker of myocardial ischemia-reperfusion injury

Examples

Experimental program
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Effect test

Embodiment 1

[0033] To establish a mouse model of ischemia-reperfusion injury and detect the level of GSDMD, the specific operations are as follows:

[0034] (1) Establishment of the mouse model of ischemia-reperfusion injury: After the mouse was anesthetized with 2% isoflurane, the chest area was depilated, sterilized with 75% alcohol, spread a towel, and a small Xmm opening was made at the X rib, and blunt dissection Muscle, stretch the ribs and extrude the heart, ligate the coronary artery at Xmm below the left atrial appendage with 6-0 silk thread, tie a slipknot, suture the incision with 4-0 silk thread, check the heart for ischemia by electrocardiogram, loosen the slipknot after 40 minutes, and observe the electrocardiogram Whether to reperfuse.

[0035] (2) Detection of GSDMD level, specifically:

[0036] Western blot: The mouse heart samples were removed and frozen in liquid nitrogen, and transferred to -80°C for storage after 24 hours. After the tissue was shredded, RIPA lysate ...

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Abstract

The invention discloses an application of GSDMD as an early diagnosis marker of myocardial ischemia-reperfusion injury, and particularly discloses a diagnostic reagent or a kit for detecting myocardial ischemia-reperfusion injury prepared from GSDMD. The GSDMD is remarkably increased after myocardial ischemia reperfusion is verified at animal and human levels, so that the myocardial ischemia reperfusion injury can be diagnosed and predicted by detecting the level of the GSDMD in peripheral blood or in-vitro myocardial tissue samples of a tested individual, and a new way is provided for early diagnosis of the myocardial ischemia reperfusion injury.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to GSDMD as a marker for early diagnosis of myocardial ischemia-reperfusion injury. Background technique [0002] In recent years, with the advent of an aging society, the improvement of people's quality of life and the widespread prevalence of cardiovascular disease (Cardiovascular Disease, CVD) risk factors, the incidence and mortality of CVD in my country have increased year by year. According to the latest "China Cardiovascular Disease Report 2016" released by the National Cardiovascular Center, CVD deaths account for the first cause of death among urban and rural residents in my country, with 45.01% in rural areas and 42.61% in cities, higher than tumors and other systemic diseases ( figure 1 ). Among them, ischemic heart disease (Ischemic Heart Disease, IHD), especially coronary heart disease (Coronary Heart Disease, CHD) is the leading cause of CVD death. Acute myocardi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/47G01N2800/32G01N2800/7019
Inventor 葛均波石怀瑞高旸孙爱军董震杨继娥高日峰
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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