One-step rooting and transplanting technique of tissue cultured seedlings of Mandshurica mandshurica

A technology, water koji technology, applied in the field of high-efficiency propagation technology of ash, can solve problems such as no systematic rooting and transplanting technology.

Active Publication Date: 2022-04-01
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology

The tissue culture of Mandshurica mandshurica mainly obtains adventitious buds through organogenesis (buds, stem segments, hypocotyls), and in order to realize large-scale reproduction, adventitious buds need to be rooted and transplanted. There are only sporadic reports on rooting and transplanting, and no research on systematic rooting and transplanting techniques

Method used

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  • One-step rooting and transplanting technique of tissue cultured seedlings of Mandshurica mandshurica
  • One-step rooting and transplanting technique of tissue cultured seedlings of Mandshurica mandshurica
  • One-step rooting and transplanting technique of tissue cultured seedlings of Mandshurica mandshurica

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Experimental program
Comparison scheme
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Embodiment Construction

[0011] 1. Effects of four basic media on the growth of Mandshurica mandshurica seedlings

[0012] Adventitious shoots induced by Mandshurica mandshurica callus were inoculated in MS, WPM, B5, IS medium supplemented with 2.0 mg / L 6-BA, 20 g / L sucrose and 7 g / L agar after elongation culture for 28 days. 30 bottles were repeated for each treatment, and one explant was inoculated in each bottle. After 30 days, the plant height, stem diameter, leaf state (leaf number and leaf color) and survival rate were counted.

[0013] Table 1 The effect of the basic medium on the strong seedlings of Mandshurica mandshurica tissue culture seedlings

[0014]

[0015]

[0016] Note: Lowercase letters represent significant differences at the P<0.05 level.

[0017] 2. The influence of the growth state of adventitious branches on the strong seedlings of Mandshurica mandshurica tissue culture seedlings

[0018] Under sterile conditions, semi-lignified single plant seedlings (with callus at t...

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Abstract

The present invention provides a kind of technique of the integration of rooting and transplanting of Mandshurica mandshurica tissue culture seedlings. First, the semi-lignified adventitious shoots obtained by tissue culture are placed in strong seedling medium (WPM+2.0mg / L 6-BA+ 20g / L sucrose+7g / L agar) after culturing for 30d, directly planted into the matrix (peat soil: vermiculite) containing rooting medium (WPM+1.4mg / L IBA+0.7mg / L NAA, without sucrose and agar) =3:1) in a seedling-growing bag, then sealed with a plastic pull bag, cultivated under specific light, temperature and humidity conditions, opened the bag mouth after 7 days, removed the bag after 14 days, and after 30 days of constant temperature and humidity cultivation, adventitious branches took root and grew out. The survival rate of new leaves is 63.33%. The invention simplifies rooting and transplanting into one step, which is one of the key technologies for rapid propagation of Mandshurica mandshurica seedlings, saves time and cost, and provides a large-scale method for the rapid propagation of Mandshurica mandshurica. Production has laid a solid foundation.

Description

Technical field: [0001] The invention belongs to the field of genetic breeding of forest trees, the field of plant tissue culture, and high-efficiency propagation technology of Mandshurica mandshurica Background technique: [0002] Fraxinus mandshurica seeds have the characteristics of deep dormancy, the dormancy period is as long as several months, the vernalization treatment time is longer, and the reproduction speed is slow, so it is particularly important to establish an efficient reproduction technology. At the same time, in order to maintain the traits of excellent varieties, shorten the breeding time, and expand the reproduction coefficient, asexual reproduction is also the best choice. The tissue culture of Mandshurica mandshurica mainly obtains adventitious buds through organogenesis (buds, stem segments, hypocotyls), and in order to realize large-scale reproduction, adventitious buds need to be rooted and transplanted. There are only sporadic reports on rooting an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G17/00A01G24/15A01G24/28
CPCA01H4/001A01H4/008A01G17/005A01G24/28A01G24/15
Inventor 詹亚光于磊丁一巍
Owner NORTHEAST FORESTRY UNIVERSITY
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