One-step rooting and transplanting technique of tissue cultured seedlings of Mandshurica mandshurica

Abstract

The present invention provides a kind of technique of the integration of rooting and transplanting of Mandshurica mandshurica tissue culture seedlings. First, the semi-lignified adventitious shoots obtained by tissue culture are placed in strong seedling medium (WPM+2.0mg / L 6-BA+ 20g / L sucrose+7g / L agar) after culturing for 30d, directly planted into the matrix (peat soil: vermiculite) containing rooting medium (WPM+1.4mg / L IBA+0.7mg / L NAA, without sucrose and agar) =3:1) in a seedling-growing bag, then sealed with a plastic pull bag, cultivated under specific light, temperature and humidity conditions, opened the bag mouth after 7 days, removed the bag after 14 days, and after 30 days of constant temperature and humidity cultivation, adventitious branches took root and grew out. The survival rate of new leaves is 63.33%. The invention simplifies rooting and transplanting into one step, which is one of the key technologies for rapid propagation of Mandshurica mandshurica seedlings, saves time and cost, and provides a large-scale method for the rapid propagation of Mandshurica mandshurica. Production has laid a solid foundation.

Description

Technical field: [0001] The invention belongs to the field of genetic breeding of forest trees, the field of plant tissue culture, and high-efficiency propagation technology of Mandshurica mandshurica Background technique: [0002] Fraxinus mandshurica seeds have the characteristics of deep dormancy, the dormancy period is as long as several months, the vernalization treatment time is longer, and the reproduction speed is slow, so it is particularly important to establish an efficient reproduction technology. At the same time, in order to maintain the traits of excellent varieties, shorten the breeding time, and expand the reproduction coefficient, asexual reproduction is also the best choice. The tissue culture of Mandshurica mandshurica mainly obtains adventitious buds through organogenesis (buds, stem segments, hypocotyls), and in order to realize large-scale reproduction, adventitious buds need to be rooted and transplanted. There are only sporadic reports on rooting an...

AI Technical Summary

Problems solved by technology

The tissue culture of Mandshurica mandshurica mainly obtains adventitious buds through organogenesis (buds, stem segments, hypocotyls), and in order to realize large-scale reproduction, adventitious buds need to be rooted and transplanted. There are only sporadic reports on rooting and transplanting, and no research on systematic rooting and transplanting techniques

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