Small microfluidic single-cell encapsulation instrument

A microfluidic, single-cell technology, applied in the field of mechanical devices, can solve problems such as high usage costs and high prices, and achieve the effects of reducing production costs, reducing manufacturing difficulty, and compact design logic.

Pending Publication Date: 2020-10-13
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the high price and high cost of use make many researchers urgently need its substitutes.

Method used

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  • Small microfluidic single-cell encapsulation instrument
  • Small microfluidic single-cell encapsulation instrument
  • Small microfluidic single-cell encapsulation instrument

Examples

Experimental program
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Embodiment Construction

[0031] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0032] like figure 2 As shown, it is an axonometric view of the entire instrument. The single-cell encapsulation platform 100 of the present invention includes: a mechanical unit 010, a microfluidic chip unit 020, a circuit unit 030, and a frame 011. The peripheral size of the present invention is 145*89* 103 (unit: mm), assembled by split design and bolt connection, wherein the mechanical modules are symmetrically placed on both sides of the device, the connection module is placed on the upper part of the device, and the microfluidic chip is placed in the middle of the connection module and the base. The circuit module is placed at the bottom of the device, and the air control pipeline and circuit lines are not marked in the figure, which does not affect the structural design. In addition, there are four injection needles, and only three nee...

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PUM

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Abstract

The invention discloses a small microfluidic single-cell encapsulation instrument. The instrument comprises a microfluidic chip module, a mechanical module, a shell assembly and an electric control module, wherein the microfluidic chip module comprises a connecting module with a liquid storage chamber, an upper cover with a bulge matched with the connecting module, and a microfluidic chip; the shell assembly comprises a base with a special structure; and the electric control module comprises a power supply, a micro-processing chip, a motor driver and a circuit board. The invention provides a single-cell packaging platform which is low in price, easy and convenient to operate and high in automation degree. According to the invention, a sample injection needle driven by a precise stepping motor is used, so the use of an air pump is avoided, precision similar to the precision of a micro-injection pump can be maintained, and the production cost of the instrument is reduced; and an integrated and modular kit is adopted, so the whole design logic is compact, more accurate closed-loop control is realized, and the manufacturing difficulty of the instrument is reduced.

Description

technical field [0001] The invention relates to the technical field of mechanical devices in the C12M biological field, in particular to a small microfluidic single-cell encapsulation instrument. Background technique [0002] With the rapid development of life sciences in recent years, sequencing technology has gradually entered everyone's field of vision. From the mapping of cell diversity maps in complex tissues to the planning and implementation of precision medicine, the development of sequencing technology is behind the scenes. However, due to the inherent defects of manual operation, if only one cell is analyzed at a time, it is obviously not enough for the development of life sciences. In recent years, two teams at Harvard University have introduced microfluidic technology into single-cell RNA-Seq methods and developed Drop-seq and inDROP respectively. In 2015, the two approaches were published in the same issue of the journal Cell. The emergence of these two method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12M1/00C12M1/36C12M1/34
CPCB01L3/5027
Inventor 瞿昆方靖文鲍宇纵丹丹
Owner UNIV OF SCI & TECH OF CHINA
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