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Caps marker for detection of melon blight resistance and its application

A melon, labeling technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of lack of molecular markers, etc.

Active Publication Date: 2022-08-02
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no disease-resistant gene has been mapped or cloned, and no molecular markers closely linked to disease-resistant genes have been reported (currently reported melon molecular markers are basically focused on agronomic traits, powdery mildew resistance, etc.)

Method used

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  • Caps marker for detection of melon blight resistance and its application
  • Caps marker for detection of melon blight resistance and its application
  • Caps marker for detection of melon blight resistance and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: This example is about the linked SNP sites and markers of the melon anti-blight gene and the method for obtaining the same.

[0029] 1. Test melon material: the present invention takes the susceptible variety E31 bred in this laboratory as the female parent (P 1 ), with the resistant variety ZQK9 as the male parent (P 2 ), using two parents to prepare F 1 , BC 1 P 1 , BC 1 P 2 and F 2 group.

[0030] Tested strains: The strains used in this experiment were isolated from diseased melon plants in the Hainan field, and were identified as Phytophthora capsicum.

[0031] 2. Analysis methods and results

[0032] (1) Analysis of disease resistance at seedling stage: The disease resistance was identified by the method of root irrigation. When the seedlings grow to the two-leaf and one-heart stage, a 1cm-deep hole is made about 1cm away from the root of the seedling, and the concentration of 1ml is 10 6 Saturated sub-suspension of 1 / m1 was injected into the w...

Embodiment 2

[0060] Example 2: F 2 Genotype verification of population and natural population materials

Embodiment approach

[0062] 1. Experimental materials

[0063] Tested melon materials: resistant parent ZQK9, susceptible parent E31, containing 497 individual plants of F 2 swarms, and a natural swarm material consisting of 40 melon materials.

[0064] 2. Experimental method

[0065] The phenotypes of all experimental materials were identified by root irrigation method. The DNA of all individual plants was extracted by CTAB method. After PCR amplification using CAPS marker, restriction endonuclease Fau I was used to digest, and polyacrylamide gel electrophoresis was used. detect ( figure 1 ). Analysis of F using CAPS markers 2 genotype of the population, combined with F 2 The identification results of disease resistance of individual plants in the field were analyzed by linkage genetic analysis using JoinMap4.0 software.

[0066] 3. Experimental results

[0067] Use the CAPS marker to pair F 2 The genotypes of 497 individual plants in the population were amplified and analyzed with JoinMa...

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PUM

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Abstract

The present invention provides a CAPS marker closely linked with the melon anti-blight gene, characterized in that the polymorphism site of the CAPS marker is located at the position of chromosome 22,902,391 of chromosome 12 in the version 3.5.1 of the melon genome, which is a G / C polymorphism The present invention also provides the application of the above molecular markers in melon breeding, variety identification and preparation of corresponding kits.

Description

technical field [0001] The present application belongs to the technical field of agricultural molecular biology detection. Specifically, the present application provides a CAPS marker closely linked with the melon anti-blight gene and its corresponding primer, a detection method and application. Background technique [0002] Melon is a crop of Cucumis melo L., an important horticultural crop in my country. According to the statistics of the United Nations Food and Agriculture Organization, my country ranks first in the world in terms of melon cultivation area and yield, which has played an important role in improving farmers' income and promoting the reform of agricultural supply-side structure. [0003] Blight is a devastating soil-borne disease caused by Phytophthora infection. It can be transmitted through rain, soil and air. The high temperature and high humidity environment can promote the occurrence and prevalence of blight. Phytophthora can infect the roots, stems, l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 王平勇徐志红徐永阳付楠赵光伟贺玉花孔维虎张健
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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