Molecular marker SNP#2 for identifying powdery mildew resisting phenotype of vigna radiata, and primer and application of molecular marker SNP#2.

A molecular marker and anti-powdery mildew technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem that molecular markers closely related to resistance have not been reported.

Active Publication Date: 2020-10-27
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, specific molecular markers close...

Method used

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  • Molecular marker SNP#2 for identifying powdery mildew resisting phenotype of vigna radiata, and primer and application of molecular marker SNP#2.
  • Molecular marker SNP#2 for identifying powdery mildew resisting phenotype of vigna radiata, and primer and application of molecular marker SNP#2.
  • Molecular marker SNP#2 for identifying powdery mildew resisting phenotype of vigna radiata, and primer and application of molecular marker SNP#2.

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Experimental program
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Embodiment 1

[0025] Example 1 CN60 X RUM5 F2 population 190 strains, CN60XRUM5 BC1F1 population 74 strains, population construction and phenotypic identification

[0026] Using the wild powdery mildew-resistant mung bean resource RUM5 as the male parent and the powdery mildew-susceptible mung bean resource CN60 as the female parent, hybrids were prepared, and an F2 segregation population containing 190 individuals was constructed. Each F2 individual plant obtained the corresponding F2 by selfing : 3 families were identified for resistance to powdery mildew. In addition, the wild powdery mildew-resistant mung bean resource RUM5 was used as the male parent, and the powdery mildew-susceptible mung bean resource CN60 was used as the female parent to prepare hybrids, and a BC1F1 segregation population containing 74 individuals was constructed, and each individual plant was self-crossed to obtain a BC1F1: 2 family , carry out powdery mildew resistance identification, two groups of synchronous te...

Embodiment 2

[0037] Extract the mung bean powdery mildew susceptible materials KPS1, Sulu 1, Sulu 3, powdery mildew resistant materials V4718, Sulu 2, V2802 and V2709, and the foreign mung bean wild species TC1966 genomic DNA, and use this molecule to mark the primer of SNP#2 (SEQ ID NO.1 / SEQ ID NO.2 and SEQ ID NO.3) were amplified by PCR for powdery mildew resistance identification, the results are shown in Table 2, and the results showed that the identification efficiency of the marker was 100%.

[0038] Table 2

[0039]

[0040] The identification of mung bean powdery mildew resistance by the above molecular markers can predict the disease resistance of mung bean plants, which can improve the breeding process of mung bean powdery mildew resistant varieties in my country.

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Abstract

The invention discloses a molecular marker SNP#2 for identifying powdery mildew resisting phenotype of vigna radiata, and a primer and application of the molecular marker SNP#2. A primer pair of the molecular marker SNP#2, namely SEQID NO.1 and SEQID NO.3, can amplify a characteristic strip of 418bp in vigna radiata varieties susceptible to powdery mildew, and cannot amplify the characteristic strip in the vigna radiata varieties resisting powdery mildew; and a primer pair of the molecular marker SNP#2, namely SEQID NO.2 and SEQID NO.3, can amplify a characteristic strip of 418bp in vigna radiata varieties resisting powdery mildew, and cannot amplify the characteristic strip in the vigna radiata varieties susceptible to powdery mildew. The powdery mildew resisting site of the vigna radiatais positioned, so that the inventor finds the molecular marker which is jointly separated from a vigna radiata gene for resisting powdery mildew, and the marker selecting efficiency achieves 100%. The molecular marker primer is used for identifying or screening powder mildew resistance of the vigna radiata. The method is quick and simple, high in selecting efficiency, and low in cost.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a molecular marker SNP#2 for identifying mung bean powdery mildew resistance phenotype, primers and applications thereof. Background technique [0002] Mung bean (Vigna radiata) is a legume, Papilionaceae Vigna plant, is an important socio-economic crop in Asia, especially in South Asia and Southeast Asia. Widely grown in India, China, Myanmar, Thailand, Bangladesh, Pakistan, Cambodia, Indonesia, Philippines and Australia. Mung bean is usually grown alone after rice, wheat and corn, or as a component of various planting systems, such as intercropping with sugarcane, because mung bean grows fast and matures early, only about 75 days. It is estimated that mung bean is produced on more than 6 million hectares. India, Myanmar and China are the main growing countries. The average yield of mung beans is not high. Diseases are one of the main causes of low yields. Common diseases th...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 薛晨晨塞班陈新袁星星陈景斌普拉给特宋塔披拉沙斯乃文闫强吴然然张红梅陈华涛崔晓艳刘晓庆顾和平
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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