Application of host defense peptide or composition thereof in preparation of drugs for treating tumors
A technology of anti-tumor drugs and compositions, applied in the field of biomedicine to achieve obvious anti-tumor effects
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Embodiment 1
[0068] MTT assay of caerin 1.1 and mutant caerin 1.1 and caerin 1.9 inhibiting tumor cell growth in vitro
[0069] 5×10 3 Place TC-1 cells or B16 cells in 96-well cell culture plates, add P3, caerin 1.1, mutant caerin 1.1 polypeptide or caerin 1.9 polypeptide at a final concentration of 5, 10, and 15 μg / ml respectively, in 10% calf serum PRMI1640 medium at 37 °C, 5% CO 2 Incubate overnight. The growth of TC-1 (left) and B16 cells (right) was detected by MTT method.
[0070] The results showed that the growth inhibitory effect of mutant caerin 1.1 on TC-1 and B16 was better than that of caerin 1.1.
Embodiment 2
[0072] Effect of triple therapy on Arg1-negative tumor-infiltrating macrophages
[0073] Experimental method: C57 / BL6 mice were subcutaneously inoculated with 5×10 5 TC-1 tumor cells. Three days after tumor inoculation, TC-1 tumor-bearing mice were divided into three groups. 3 mice per group. One group is the control group. The mice are immunized with the vaccine, locally injected with caerin polypeptide in the tumor, and injected with equal volume of PBS of PD-1 antibody. 3 days and 9 days after tumor inoculation, 100 μl of vaccine containing 40 μg Ex (10 μg for each polypeptide) / 10 μg MPLA / 300 μg anti-IL-10 receptor antibody vaccine was injected twice subcutaneously. 300 μg PD-1 antibody twice. On days 3-9 after tumor inoculation, 30 μg of Caerin 1.1 and 1.9 (the weight ratio of Caerin 1.1 and 1.9 in the treatment group was 1:1) or 30 μg of control peptide P3 (GTELPSPPSVWFEAEFK-OH) (control group) were locally injected into the tumor for 7 consecutive days. sky. A grou...
Embodiment 3
[0078] Triple therapy reduces the number of tumor-infiltrating B cells
[0079] Experimental method is the same as embodiment 2.
[0080]The B cell population is the major tumor-associated cell population, accounting for 4.84% of the total number of CD45+ cells isolated from untreated TC-1 tumors. In contrast, the proportion of B cells decreased to 1.61% (87 cells) and 0.26% (15 cells) in the P3-treated and caerin 1.1 / 1.9-treated groups, respectively. GO analysis of B cells revealed enriched biological processes including activation (corrected P value = 9.95E-15), differentiation (corrected P value = 8.27E-07) and proliferation (corrected P value = 3.49E- 10) B cells, which regulate B cell receptor signaling (corrected P value = 3.53E-04) ( image 3 A). KEGG enrichment analysis also revealed that the B cell receptor signaling pathway was the second most important pathway after the ribosome (P value = 5.76E-06), while antigen processing and presentation was also enriched (P ...
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