Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of monkey earring polypeptide and preparation method of monkey earring polypeptide liposome

A technology of monkey earrings and peptide lipids, which is applied in the preparation of monkey earring polypeptides and monkey earring polypeptide liposomes, can solve the problems of low yield of monkey earring polypeptides, and solve the problems of easy dissolution and high encapsulation rate , the effect of improving solubility

Active Publication Date: 2021-10-12
广东态合堂实业有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction of monkey earrings is mostly organic matter extraction. For example, the Chinese invention patent application number 2013103140324 discloses the preparation method of monkey earring extracts. Extraction is obtained by extracting with water or ethanol or ethanol aqueous solution. However, this preparation method monkey The yield of earring polypeptide is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of monkey earring polypeptide, comprising the following steps:

[0025] S1, take the monkey earrings, and move them to the fermenter after sterilization;

[0026] S2. After adding yeast powder, lactic acid bacteria powder and appropriate amount of water to the fermenter, the yeast powder and lactic acid bacteria powder are dissolved in water in advance, and sealed for 72 hours of fermentation; the weight of yeast powder is 0.3% of the weight of monkey earrings, and the weight of lactic acid bacteria powder is monkey 0.1% of the weight of the earrings;

[0027] S3. Add cellulase and pectinase to water of 5 times the weight of the monkey earrings respectively, vacuum the enzyme liquid into the fermenter, and keep the mixed solution in the fermenter at 40° C. for 18 hours. The addition amount is 0.012% of the monkey earring weight, cooled to room temperature;

[0028] S4, adding tartaric acid to the fermenter to adjust the pH of the mixed solution to...

Embodiment 2

[0037] A preparation method of monkey earring polypeptide, comprising the following steps:

[0038] S1, take the monkey earrings, and move them to the fermenter after sterilization;

[0039] S2. After adding yeast powder, lactic acid bacteria powder and appropriate amount of water to the fermenter, the yeast powder and lactic acid bacteria powder are dissolved in water in advance, and sealed and fermented for 108 hours; the weight of yeast powder is 0.1% of the weight of monkey earrings, and the weight of lactic acid bacteria powder is monkey 0.1% of the weight of the earrings;

[0040] S3. Add cellulase and pectinase to water with 4 times the weight of monkey earrings respectively, vacuum the enzyme liquid into the fermenter, and keep the mixed solution in the fermenter at 50° C. for 36 hours. The addition amount is 0.012% of the monkey earring weight, cooled to room temperature;

[0041] S4, adding tartaric acid to the fermenter to adjust the pH of the mixed solution is 3,...

Embodiment 3

[0050] A preparation method of monkey earring polypeptide, comprising the following steps:

[0051] S1, take the monkey earrings, and move them to the fermenter after sterilization;

[0052] S2. After adding yeast powder, lactic acid bacteria powder and appropriate amount of water to the fermenter, the yeast powder and lactic acid bacteria powder are dissolved in water in advance, sealed and fermented for 72 hours; the weight of yeast powder is 0.1% of the weight of monkey earrings, and the weight of lactic acid bacteria powder is monkey earrings 0.1% of the weight of the earrings;

[0053]S3. Add cellulase and pectinase to water of 5 times the weight of the monkey earrings respectively, vacuum the enzyme liquid into the fermenter, and keep the mixed solution in the fermenter at 40° C. for 18 hours. The addition amount is 0.01% of the monkey earring weight, cooled to room temperature;

[0054] S4, adding tartaric acid to the fermenter to adjust the pH of the mixed solution t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a monkey earring polypeptide, which comprises the following steps: S1, taking the monkey earring, and moving it to a fermenter after sterilization; S2, adding yeast powder, lactic acid bacteria powder and an appropriate amount of water into the fermenter, sealing the Fermentation; S3, adding cellulase and pectinase to water 4 to 5 times the weight of monkey earrings respectively, vacuuming the enzyme solution into the fermenter, and keeping the mixed solution in the fermenter at 40-50°C for 18-36 hours; S4, adding acid solution to the fermenter to adjust the pH of the mixed solution to 3-5, and filtering to obtain a filtrate; S5, concentrating and drying the filtrate to obtain dry powder of the monkey earring polypeptide. Destroy the cell wall of monkey earrings by enzymatic hydrolysis, avoiding the adsorption of monkey earring polypeptides and polyphenols on the cell walls to affect the yield, and using the surfactant Tween 80 to solve the problem that liposomes are difficult to form films and dissolve easily, and the obtained monkey earrings The encapsulation efficiency of earring polypeptide liposome is high.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine pharmacy, in particular to a preparation method of monkey earring polypeptide and a preparation method of monkey earring polypeptide liposome. Background technique [0002] Earrings, the scientific name is the bisalis tree, and the folks are also called the popping tree, the heart tree, the three wrongs, the pissing bucket, the hair-washing tree, and the money on the ground. It is a perennial arboreal plant. The original plant was identified as Mimosaceae, which was later classified as Fabaceae and Monkey Earring by the World Health Organization, and its scientific name is Pithecellobium clypearia (perennial arbor tree). Monkey earrings are bitter and cold in nature, clear away heat and detoxify, reduce dampness and sores, and are unique southern medicinal materials for treating various heat-toxin symptoms. [0003] At present, the extraction of monkey earrings is mostly organic matter...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P21/00C07K1/34A61K9/127A61K47/26A61K38/01A61P11/00A61P31/00A61P11/14
CPCA61K9/127A61K38/011A61K47/26A61P11/00A61P11/14A61P31/00C07K1/34C12P21/00C12P39/00
Inventor 陈东明孙葵英龙孝军
Owner 广东态合堂实业有限公司