Single-cell temperature detection sensor and manufacturing method and detection method thereof

Abstract

The invention provides a single-cell temperature detection sensor and a manufacturing method and a detection method thereof. The sensor comprises a glass substrate (1) and a PDMS sheet (2) which are attached to each other, a Pt working electrode block (3) for electric conduction and temperature sensing and an outgoing line (4) of the Pt working electrode block (3) are sputtered on the binding surface of the glass substrate (1); a flow groove (7) is formed in the binding surface of the PDMS sheet (2); a flow channel is formed on the glass substrate (1) after lamination; a sample inlet hole (5)and a sample outlet hole (6) are formed in the two ends of the flow groove (7), an open box dam for single cells to flow in and remain is arranged in the flow groove (7) and located above the Pt working electrode block (3), an opening of the open box dam faces the sample injection direction, and a height gap for sample fluid and air to flow through during detection is reserved between the open boxdam and the Pt working electrode block and is smaller than a to-be-detected single cell. According to the invention, tiny temperature fluctuation in the single-cell vital movement process can be detected, and online continuous monitoring of temperature indexes can be realized.

Description

technical field [0001] The invention relates to the technical field of cell detection, in particular to a single-cell temperature detection sensor, a manufacturing method thereof, and a corresponding single-cell temperature detection method. Background technique [0002] In the field of biology, temperature is one of the indispensable indicators in the field of life sciences to study the life process of organisms. In life science research, on the one hand, various physiological and chemical reactions are constantly going on in living cells, and the external manifestation of energy changes closely related to these reactions is the change of temperature. On the other hand, in response to external stimuli, cells rapidly adjust their metabolic activities in response to environmental changes, which are also accompanied by significant temperature changes. Therefore, it is of great significance to realize the real-time detection of single cell temperature, whether it is for the st...

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a single-cell temperature detection sensor, which integrates single-cell capture, lysis, and temperature detection functions, and solves the problems of complex chip structure and cumbersome steps in the prior art.

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