Multichannel electrospray micro-fluidic chip and application thereof

Abstract

The invention discloses a multichannel electrospray micro-fluidic chip and application thereof. The multichannel electrospray micro-fluidic chip comprises two first gas phase flow channels and at least two liquid phase flow channels, wherein the liquid phase flow channels are arranged between the two first gas phase flow channels, and the nozzles of the first gas phase flow channels and nozzles ofthe liquid phase flow channels are arranged at intervals. In an electrospray process, due to the fact that the relative positions of the liquid phase nozzle and the first gas phase nozzle are fixed and the spray conditions are stable and consistent, the macroscopic electrospray extraction function can be achieved, the extraction efficiency and the extraction effect of extraction liquid on to-be-detected liquid are improved, and rapid detection of complex samples is facilitated; and the problem that double liquid paths or multiple liquid paths are difficult to electrospray at the same time issolved, finally more application forms are created for combination of a micro-fluidic chip and a mass spectrometer, and the micro-fluidic chip can be widely applied to the aspects of rapid substance mixing reaction, protein folding and stretching analysis, internal standard test and the like.

Description

technical field [0001] The invention relates to the field of microfluidic chips used for electrospraying, in particular to a multi-channel electrospraying microfluidic chip and its application. Background technique [0002] For mass spectrometry, single-channel sample ionization has been difficult to meet the needs of related tests, and dual-channel or even multi-channel ionization has become a research hotspot. Dual-channel or multi-channel spray ion sources can realize various applications, such as accurate determination of mass through dual-channel internal standards, extraction and detection of complex samples by dual-channel spraying, and multi-channel spraying to enhance signal intensity and improve sensitivity. It is impossible to achieve with a single spray. [0003] In the application research of dual spray, extractive electrospray ion source (EESI) plays an important role in mass spectrometry. Because when complex samples such as urine, serum, lake water, and mil...

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Problems solved by technology

[0004] However, when EESI is used for dual extraction spray, the placement and angle of the two nozzles will affect the stability and sensitivity of the test, which usually leads to low extraction efficiency and poor ionization effect of the liquid to be tested, resulting in weak test signals , poor experimental operability and reproducibility

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