Traditional Chinese medicine compound composition with anti-colon cancer effect and application thereof
A composition and technology for colon cancer, applied in the directions of drug combinations, pharmaceutical formulations, anti-tumor drugs, etc., can solve the problems of poor income, difficult treatment of colon cancer, and high treatment costs, and achieve the goal of inhibiting the growth of colon cancer and inhibiting colon cancer. Effects of cancer growth, inhibition of proliferation and migration
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preparation example Construction
[0062] The preparation method of above four kinds of prescriptions all implements according to the following method:
[0063] (1) Take the crude drug raw material of each dose of medicinal material of the compound Chinese medicine composition, add water of 10 times the weight of the crude drug, after boiling, filter out the first medicinal liquid after decocting for 50 minutes; add 9 times the weight of the crude drug amount of water, decocting for 45 minutes, and then filter out the second medicinal solution; then add water with an amount 8 times the weight of the raw material drug, and decoct for 40 minutes, and then filter out the third medicinal solution;
[0064] (2) Combine the first medicinal liquid, the second medicinal liquid, and the third medicinal liquid prepared in the aforementioned step (1) to obtain the crude extract of traditional Chinese medicine, decoct and condense it to a thick shape, that is, the concentrated Chinese medicinal liquid;
[0065] (3) Take th...
Embodiment 1
[0067] Example 1: The provided traditional Chinese medicine compound inhibits colon cancer cell proliferation in vitro and has low cytotoxicity.
[0068] Technical method:
[0069] 1. Cell culture
[0070] Human colon cancer cells HCT116 and HT29 were cultured in DMEM medium containing 10% fetal bovine serum and 1% penicillin-streptomycin double antibody, human normal liver cell L-02 and human normal colonic epithelial cell NCM460 were cultured in 10% In the 1640 medium of fetal bovine serum and 1% penicillin-streptomycin double antibody, human normal adrenal fibroblast HAF was cultured in 10% fetal bovine serum, 1% penicillin-streptomycin double antibody and 1% glutamine in DMEM medium. All cells were cultured in a constant temperature incubator at 37°C (humidity 95%, CO 2 Concentration 5%).
[0071] 2. Determination of cell proliferation by sulforhodamine B (SRB) method
[0072] Cells in the logarithmic phase were digested at different densities (colon cancer cells 5×10...
Embodiment 2
[0074] Example 2: The provided traditional Chinese medicine compound inhibits colon cancer cell colony formation.
[0075] Technical method:
[0076] 1. Colony formation experiment
[0077] Insert 2000 colon cancer cells into a 6-well plate, add different concentrations of the traditional Chinese medicine compound of the present invention after the cells adhere to the wall, fix the cells with paraformaldehyde after 10 days, then stain with crystal violet, take pictures and count the number of clones, each Group experiments were repeated three times.
[0078] Experimental results such as figure 2 As shown in (A and B), the provided traditional Chinese medicine compound inhibits colon cancer cell HCT116 colony formation in a dose-dependent manner.
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