Intergeneric distant hybrid creation method of crossostephium chinense and chamomile

A technology of distant hybrids and Hibiscus chrysanthemum, applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., can solve the problems of small immature embryos, difficult to peel off, and few applications, and achieve the effect of avoiding premature aging of embryos

Inactive Publication Date: 2020-11-17
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Embryo rescue, as an important means to overcome the barriers of distant hybridization, has been applied in Chrysanthemum, but because Hibiscus h

Method used

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  • Intergeneric distant hybrid creation method of crossostephium chinense and chamomile
  • Intergeneric distant hybrid creation method of crossostephium chinense and chamomile
  • Intergeneric distant hybrid creation method of crossostephium chinense and chamomile

Examples

Experimental program
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Effect test

Embodiment 1

[0082] Example 1 Using Immature Embryo Rescue Technology to Obtain the Intergeneric Hybrid Offspring of Hibiscus Chrysanthemum and Chamomile

[0083] Utilize the immature embryo rescue technique to obtain the intergenus hybrid progeny of hibiscus chrysanthemum and chamomile, and the method comprises the following steps:

[0084] 1) Preparation of parents: the female parent is selected from the diploid salt-tolerant wild species of Hibiscus chrysanthemum (Cr. It is a well-known public variety. Adjust the flowering period of Hibiscus chrysanthemum, and start 8-hour short-day conservation in August, so that it will reach the full flowering period in mid-October.

[0085] 2) Distant hybridization: In the Xiaotangshan Nursery of the National Flower Engineering Center, hibiscus chrysanthemum and chamomile were hybridized in the greenhouse. Two days before flowering, the female parent was artificially detasselled and bagged for isolation, and the male parent was pollinated with fre...

Embodiment 2

[0091] 6) Obtaining hybrid F1 generation plants: after the roots of the seedlings grow to more than 1 cm, the obtained hybrid F1 generation plants are opened for 2-3 days to harden the seedlings, and the seedlings are adapted to the autotrophic growth of the field by gradually reducing the relative humidity and increasing the light and a bacterial environment. Take out the plant and wash the culture medium attached to its surface, and transplant it into a plug whose matrix is ​​coarse-grained vermiculite:perlite=1:1. Routine management in pots after one month. Finally, 28 large seedlings of hybrid offspring of Hibiscus chrysanthemum and chamomile were obtained. Example 2 Using Morphological Observation and SSR Analysis to Identify the Authenticity of Hibiscus and Chamomile Hybrids

[0092] Utilize morphological observation and SSR analysis to identify the authenticity of Hibiscus chrysanthemum and Chamomile hybrids obtained in embodiment 1, said method may further comprise t...

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Abstract

The invention discloses an intergeneric distant hybrid creation method of crossostephium chinense and chamomile. The method includes the steps that artificial cross breeding is carried out by taking diploid crossostephium chinense of salt-tolerant germplasm in crossostephium chinense genera as a female parent and diploid chamomile of the salt-tolerant germplasm in chrysanthemum genera as a male parent; through in-vitro culture of young embryos, continuous development of distant hybridization embryos is guaranteed, and the problem of embryo abortion is avoided; the filial generation grows well,and can bloom normally in the adult-plant stage; and hybrid morphological observation and SSR analysis are carried out on an F1-generation material to finally obtain true hybrid progenies of the crossostephium chinense and the chamomile. According to the intergeneric distant hybrid creation method of the crossostephium chinense and the chamomile, the intergeneric distant hybridization obstacle ofthe crossostephium chinense and the chamomile is overcome, distant hybrid progenies are obtained, and a batch of new germplasm is created.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a method for creating intergenus distant hybrids of hibiscus chrysanthemum and chamomile. Background technique [0002] Breeders from all over the world are devoting themselves to excavating more excellent resources of Chrysanthemum close relatives, and have conducted a large number of hybridization attempts on many species of Chrysanthemum in a broad sense, in order to breed more abundant new germplasm. Including Chrysanthemum and Chrysanthemum (Wang Siqing, 1993), Japanese Chrysanthemum and Chrysanthemum (Ohishi, 1996), Chrysanthemum and Chrysanthemum (Abd EI-Twab et al., 1999) and so on. However, there are few related studies on the hybridization of Hibiscus and Chrysanthemum. [0003] Hibiscus chrysanthemum (Crossostephium chinense), a single genus and single species of Asteraceae, is unique to my country and is produced in the southeast coastal area. Evergreen subshrub. The ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/02A01H1/04A01H4/001A01H4/008
Inventor 孙明钟剑许婷陈俊通郭彦宏李大伟巴婷婷常丽娜张启翔程堂仁
Owner BEIJING FORESTRY UNIVERSITY
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