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Preparation method and application of passiflora edulis peel polysaccharide degraded by using lactobacillus brevis produced enzyme

A technology of Lactobacillus brevis and passionflower, applied in skin care preparations, microbial-based methods, biochemical equipment and methods, etc.

Pending Publication Date: 2020-11-20
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant report on the research on the degradation of plant polysaccharides by the enzyme produced by Lactobacillus brevis

Method used

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  • Preparation method and application of passiflora edulis peel polysaccharide degraded by using lactobacillus brevis produced enzyme
  • Preparation method and application of passiflora edulis peel polysaccharide degraded by using lactobacillus brevis produced enzyme
  • Preparation method and application of passiflora edulis peel polysaccharide degraded by using lactobacillus brevis produced enzyme

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Experimental program
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Embodiment Construction

[0023] The technical solution of the present invention will be further described below through examples.

[0024] (1) Preparation of passion fruit peel polysaccharide

[0025] Passiflora peel first removes substances such as pigments and phenols with 75% ethanol. Immerse in distilled water, the ratio of solid to liquid is 1:16g / ml, extract twice at 100°C for 3 hours, combine the extracts to concentrate, centrifuge the concentrated solution at 4000rpm for 15min, and precipitate the supernatant with alcohol until the ethanol concentration is 70%, centrifuge again, The precipitate was taken and freeze-dried, and the dried sample was collected, namely the passion fruit peel polysaccharide.

[0026] (2) Lactobacillus brevis producing enzyme nor passion fruit peel

[0027] Lactobacillus brevis was used as the experimental strain, and glucose was used as the carbon source in the MRS medium. Cultured in a shaking incubator at 37°C and 120 rpm. The crude enzyme solution is separate...

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Abstract

The invention discloses an enzymolysis modification process for degrading passiflora edulis peel polysaccharide by using a lactobacillus brevis produced enzyme and an application of the passiflora edulis peel polysaccharide, and belongs to the field of medicinal food research and development. The passiflora edulis peel polysaccharide is prepared from passiflora edulis peels, lactobacillus brevis is used as an experimental strain, glucose is used as a carbon source, a crude enzyme solution is produced by culture and uniformly mixed with the polysaccharide, and enzymolysis is performed to obtainthe passiflora edulis peel polysaccharide subjected to enzymolysis modification. Results show that the passiflora edulis peel polysaccharide degraded by the lactobacillus brevis produced enzyme has better antioxidant activity, and has a wide application prospect in antioxidant health care products and cosmetics.

Description

technical field [0001] The invention relates to an enzymatic modification process for degrading passion fruit peel polysaccharides by using Lactobacillus brevis to produce enzymes and its application in the preparation of antioxidants, which belongs to the field of research and development of medicinal food. Background technique [0002] Passiflora is a herbaceous vine of the Passiflora family Passiflora. It has a comprehensive fragrance of various fruits, so it is also called passion fruit, egg fruit, etc. Because of its orange-yellow juice, bright color and particularly aromatic smell, it enjoys the reputation of "king of juice". Passiflora is suitable for growth in warm or high temperature and humid climates, but not in cold regions. It is more common in tropical and subtropical regions. [0003] Passiflora is a high-quality fruit with the same source of medicine and food. It has the functions of invigorating the spleen and appetizing, entraining qi and relieving pain, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C08B37/00A23L33/125A61K31/715A61P39/06A61K8/73A61Q19/08C12R1/24
CPCC12P19/14C12P19/04C08B37/0003A23L33/125A61K31/715A61P39/06A61K8/73A61Q19/08A23V2002/00A23V2200/30A23V2250/51
Inventor 关媛刘承鑫黄艳李霞崔萌佳
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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