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Method for extracting 5-hydroxytryptophan from griffonia seeds

A technology of pentahydroxytryptophan and Ghana seed, applied in the direction of organic chemistry, can solve the problems of low purity and low yield of pentahydroxytryptophan

Pending Publication Date: 2020-11-24
太阳树(厦门)生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic production of 5-hydroxytryptophan has disadvantages such as low yield and low purity.

Method used

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Effect test

Embodiment 1

[0021] A method for extracting five-hydroxytryptophan from Gardner seeds provided in this embodiment comprises the following steps:

[0022] S1: oil extraction, take 10kg Ghana seed raw material, press with a screw press, remove most of the fat in the Ghana seed raw material.

[0023] S2: Extraction, the slag after degreasing is extracted by countercurrent extraction method, and 100g of phosphite (TNP) is accurately weighed, which mainly plays an antioxidant role. 180L of methanol with a volume fraction of 85% is configured to dissolve the weighed TNP In methanol solution, as the extraction solvent, the extraction temperature is controlled at 45° C. to obtain 170 L of countercurrent extract solution, and the concentration of five-hydroxytryptophan (5-HTP) at the last port of countercurrent detection by HPLC is lower than 0.5 mg / ml.

[0024] S3: Coarse filtration, coarse filtration is performed on the countercurrent extract produced in step S2, specifically, a double-layer vibr...

Embodiment 2

[0034] A method for extracting five-hydroxytryptophan from Gardner seeds provided in this embodiment comprises the following steps:

[0035] S1: Oil extraction, take 5 kg of Ghana seed raw material, and press it with a screw press to remove most of the oil in the Ghana seed raw material.

[0036] S2: Extraction, the slag after degreasing is extracted by countercurrent extraction method, accurately weigh 40g of phosphite (TNP), configure 85L of methanol with a volume fraction of 86%, and dissolve the weighed TNP in methanol solution as the extraction method. Solvent, the extraction temperature is controlled at 40° C. to obtain 85 L of countercurrent extract solution, and the concentration of five-hydroxytryptophan (5-HTP) at the last port of countercurrent detection by HPLC is lower than 0.5 mg / ml.

[0037] S3: Coarse filtration, coarse filtration is performed on the countercurrent extract produced in step S2, specifically, a double-layer vibrating sieve is used for coarse filt...

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Abstract

The invention discloses a method for extracting 5-hydroxytryptophan from griffonia seeds, and belongs to the technical field of plant extraction. The method for extracting the 5-hydroxytryptophan fromthe griffonia seeds comprises the following steps: S1, oil pressing; S2, extraction: extracting the deoiled residues by adopting countercurrent extraction, wherein the extraction solvent is a methanol solution added with phosphite ester, the addition amount of the phosphite ester is 0.5-1.5% of the mass of the griffonia seed raw material, AND the extraction temperature is 40-50 DEG C; S3: coarsefiltration; S4: fine filtration; S5: vacuum concentration: adding the phosphite ester into a feed liquid subjected to the fine filtration in the S4, performing vacuum concentration until the concentrate is 1 / 7 to 1 / 5 of the bulk of the feed liquid, stopping the concentration, make up the concentrate with water which is 0.9-1.2 times by volume of the concentrate, add phosphite to the concentrate, and then continuing the concentration wherein the concentration temperature is 60-65 DEG C; S6, extracting and impurity removing; S7, solid-liquid separation; S8, refining; S9, washing with water to remove a solvent; S10, drying; and S11, crushing. The method for extracting the 5-hydroxytryptophan from griffonia seeds is high in product purity.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting five-hydroxytryptophan from Ghana seeds. Background technique [0002] Five-hydroxytryptophan (also known as 5-hydroxytryptophan, five-hydroxytryptophan) is a natural plant extract with a molecular weight of 220.23. Penta-hydroxytryptophan is mainly extracted from Ghana seed, which is the dry seed of Griffonia simplicifolia, a tropical leguminous shrub plant, which grows wild in West African countries such as Ghana, Ivory Coast and Togo. Five-hydroxytryptophan has a variety of biological effects, and is an important raw material for neurological drugs and health care drugs. It is widely used in clinical practice, has important utilization value and broad market prospects. At present, domestic production of 5-hydroxytryptophan has disadvantages such as low yield and low purity. Contents of the invention [0003] The purpose of the p...

Claims

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Application Information

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IPC IPC(8): C07D209/20
CPCC07D209/20
Inventor 陈义刘裕陈强
Owner 太阳树(厦门)生物工程有限公司
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