Domesticated cultivation method of anoectochilus roxburghii tissue culture seedlings

Abstract

The invention discloses a domesticated cultivation method of anoectochilus roxburghii tissue culture seedlings. The method comprises the following steps: (1) building a greenhouse; (2) preparing a culture medium, wherein the culture medium is prepared from sterilized soil, coco coir, river sand and medicine residues by mixing in a mass ratio of (8-10):1:(1-2):(0.1-0.2); (3) hardening seedlings inbottles: gradually uncovering the culture bottles filled with anoectochilus roxburghii tissue culture rooting seedlings in a culture room, and then carrying out transition culture for 7-10 days; (4) cleaning and sterilizing: dividing the anoectochilus roxburghii tissue culture rooting seedlings into single plants, soaking the seedlings with clear water, repeatedly changing water and soaking for 4-6 times, and then soaking the seedlings with a plant extracting solution; (5) transplanting the tissue culture seedlings: sequentially transplanting the anoectochilus roxburghii tissue culture rootingseedlings into the culture medium of a plug tray; (6), performing environment control after transplanting; (7), performing fertilization management and pest control; (8), conducting harvesting: harvesting the seedlings 5-10 months after transplanting. The domesticated cultivation technology has the higher survival rate, the survival rate can reach 90% or above, and the transplanted anoectochilusformosanus is high in growth speed, good in growth vigor, high in yield and good in quality.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for domesticating and cultivating tissue culture seedlings of A. Background technique [0002] Clematis ( Anoectochilus roxburghii ) is a perennial herbaceous plant of the genus Orchidaceae. It is a traditional precious medicinal material in my country. It has the effects of cooling and detoxifying, nourishing yin and reducing fire, reducing inflammation and relieving pain. The whole herb is used as medicine, and it has a wide range of folk treatments. "Fujian Drugs" contains: its nature and flavor are flat and sweet, and it has the effects of clearing heat and cooling blood, dispelling wind and dampness, and is mainly used for hemoptysis, bronchitis, nephritis, cystitis, diabetes, chyluria, hematuria, rheumatoid arthritis, children's Acute convulsions, poisonous snake bites, etc., are mostly used in folks for children with persistent high fever ...

AI Technical Summary

Problems solved by technology

Due to the low natural reproduction rate of wild clematis and the large amount of man-made excavation, the domestic wild clematis is almost extinct

Therefore, artificial cultivation, especially the use of tissue culture techniques to propagate A. clematis is an effective means to protect its resources. In recent years, although A. Progress, but the existing knowledge of clematis tissue culture technology blindly pursues the yield of clematis, and the excessive use of pesticides and auxins affects the quality of clematis, and the survival rate of tissue culture seedlings transplanted is still common Low, the survival rate of transplanting is about 60%, the slow seedling period is long, the probability of being infected by root rot and stem rot is high, and the transplanting of tissue culture seedlings from sterile heterotrophic to autotrophic outside the bottle During the domestication period, it is very easy to die a large number of seedlings; or the quality of the seedlings produced is not high, and the effect of field planting is poor, etc.