Domesticated cultivation method of anoectochilus roxburghii tissue culture seedlings

Abstract

The invention discloses a domesticated cultivation method of anoectochilus roxburghii tissue culture seedlings. The method comprises the following steps: (1) building a greenhouse; (2) preparing a culture medium, wherein the culture medium is prepared from sterilized soil, coco coir, river sand and medicine residues by mixing in a mass ratio of (8-10):1:(1-2):(0.1-0.2); (3) hardening seedlings inbottles: gradually uncovering the culture bottles filled with anoectochilus roxburghii tissue culture rooting seedlings in a culture room, and then carrying out transition culture for 7-10 days; (4) cleaning and sterilizing: dividing the anoectochilus roxburghii tissue culture rooting seedlings into single plants, soaking the seedlings with clear water, repeatedly changing water and soaking for 4-6 times, and then soaking the seedlings with a plant extracting solution; (5) transplanting the tissue culture seedlings: sequentially transplanting the anoectochilus roxburghii tissue culture rootingseedlings into the culture medium of a plug tray; (6), performing environment control after transplanting; (7), performing fertilization management and pest control; (8), conducting harvesting: harvesting the seedlings 5-10 months after transplanting. The domesticated cultivation technology has the higher survival rate, the survival rate can reach 90% or above, and the transplanted anoectochilusformosanus is high in growth speed, good in growth vigor, high in yield and good in quality.

Description

technical field

[0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for domesticating and cultivating tissue culture seedlings of A. Background technique

[0002] Clematis ( Anoectochilus roxburghii ) is a perennial herbaceous plant of the genus Orchidaceae. It is a traditional precious medicinal material in my country. It has the effects of cooling and detoxifying, nourishing yin and reducing fire, reducing inflammation and relieving pain. The whole herb is used as medicine, and it has a wide range of folk treatments. "Fujian Drugs" contains: its nature and flavor are flat and sweet, and it has the effects of clearing heat and cooling blood, dispelling wind and dampness, and is mainly used for hemoptysis, bronchitis, nephritis, cystitis, diabetes, chyluria, hematuria, rheumatoid arthritis, children's Acute convulsions, poisonous snake bites, etc., are mostly used in folks for children with persistent high fever ...

Examples

Embodiment 1

[0027] The domestication cultivation method of the clematis tissue culture seedling described in present embodiment 1, comprises the following steps:

[0028] ①Greenhouse construction: choose a location with open terrain, facing south and far away from pollution sources, build a steel frame greenhouse as usual, and lay sunshade nets on the roof and surroundings of the greenhouse, and install heating equipment, humidification equipment and irrigation equipment in the shed , and build a planting frame in the greenhouse at the same time;

[0029]② Preparation of cultivation substrate: the cultivation substrate is prepared by mixing sterilized soil, coconut peat, river sand and medicinal dregs in a mass ratio of 8:1:1 to 2:0.1, and the medicinal dregs are aloe vera, garlic , mulberry, honeysuckle, Huoxiang extract medicine dregs, the sterilized soil is prepared by mixing poultry manure, humus and quicklime according to the ratio of 1:1:0.1 in mass ratio, and the poultry manure is ...

Embodiment 2

[0037] The domestication and cultivation method of the tissue-cultured seedlings of Clematis clematis described in the present embodiment 2 comprises the following steps:

[0038] ①Greenhouse construction: choose a location with open terrain, facing south and far away from pollution sources, build a steel frame greenhouse as usual, and lay sunshade nets on the roof and surroundings of the greenhouse, and install heating equipment, humidification equipment and irrigation equipment in the shed , and build a planting frame in the greenhouse at the same time;

[0039] ② Preparation of cultivation substrate: the cultivation substrate is prepared by mixing sterilized soil, coconut peat, river sand and medicinal dregs according to the ratio of 9:1:1.5:0.15 in mass ratio, and the medicinal dregs are aloe, garlic, mulberry , honeysuckle, Huoxiang extract medicine dregs, the sterilized soil is prepared by mixing poultry manure, humus and quicklime according to the ratio of mass ratio 2:...

Embodiment 3

[0047] The domestication and cultivation method of the tissue cultured seedling of A. clematis described in the present embodiment 3, comprises the following steps:

[0048] ①Greenhouse construction: choose a location with open terrain, facing south and far away from pollution sources, build a steel frame greenhouse as usual, and lay sunshade nets on the roof and surroundings of the greenhouse, and install heating equipment, humidification equipment and irrigation equipment in the shed , and build a planting frame in the greenhouse at the same time;

[0049] 2. Preparation of cultivation substrate: the cultivation substrate is prepared by mixing sterilized soil, coconut peat, river sand and medicinal dregs according to the ratio of 10:1:2:0.2 in mass ratio, and the medicinal residues are aloe, garlic, mulberry , honeysuckle, Huoxiang extract medicine dregs, the sterilized soil is prepared by mixing poultry manure, humus and quicklime according to the ratio of 3:3:0.1 in mass r...

technical field

[0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for domesticating and cultivating tissue culture seedlings of A. Background technique

[0002] Clematis ( Anoectochilus roxburghii ) is a perennial herbaceous plant of the genus Orchidaceae. It is a traditional precious medicinal material in my country. It has the effects of cooling and detoxifying, nourishing yin and reducing fire, reducing inflammation and relieving pain. The whole herb is used as medicine, and it has a wide range of folk treatments. "Fujian Drugs" contains: its nature and flavor are flat and sweet, and it has the effects of clearing heat and cooling blood, dispelling wind and dampness, and is mainly used for hemoptysis, bronchitis, nephritis, cystitis, diabetes, chyluria, hematuria, rheumatoid arthritis, children's Acute convulsions, poisonous snake bites, etc., are mostly used in folks for children with persistent high fever ...

Examples

Embodiment 1

[0027] The domestication cultivation method of the clematis tissue culture seedling described in present embodiment 1, comprises the following steps:

[0028] ①Greenhouse construction: choose a location with open terrain, facing south and far away from pollution sources, build a steel frame greenhouse as usual, and lay sunshade nets on the roof and surroundings of the greenhouse, and install heating equipment, humidification equipment and irrigation equipment in the shed , and build a planting frame in the greenhouse at the same time;

[0029]② Preparation of cultivation substrate: the cultivation substrate is prepared by mixing sterilized soil, coconut peat, river sand and medicinal dregs in a mass ratio of 8:1:1 to 2:0.1, and the medicinal dregs are aloe vera, garlic , mulberry, honeysuckle, Huoxiang extract medicine dregs, the sterilized soil is prepared by mixing poultry manure, humus and quicklime according to the ratio of 1:1:0.1 in mass ratio, and the poultry manure is ...

Embodiment 2

[0037] The domestication and cultivation method of the tissue-cultured seedlings of Clematis clematis described in the present embodiment 2 comprises the following steps:

[0038] ①Greenhouse construction: choose a location with open terrain, facing south and far away from pollution sources, build a steel frame greenhouse as usual, and lay sunshade nets on the roof and surroundings of the greenhouse, and install heating equipment, humidification equipment and irrigation equipment in the shed , and build a planting frame in the greenhouse at the same time;

[0039] ② Preparation of cultivation substrate: the cultivation substrate is prepared by mixing sterilized soil, coconut peat, river sand and medicinal dregs according to the ratio of 9:1:1.5:0.15 in mass ratio, and the medicinal dregs are aloe, garlic, mulberry , honeysuckle, Huoxiang extract medicine dregs, the sterilized soil is prepared by mixing poultry manure, humus and quicklime according to the ratio of mass ratio 2:...

Embodiment 3

[0047] The domestication and cultivation method of the tissue cultured seedling of A. clematis described in the present embodiment 3, comprises the following steps:

[0048] ①Greenhouse construction: choose a location with open terrain, facing south and far away from pollution sources, build a steel frame greenhouse as usual, and lay sunshade nets on the roof and surroundings of the greenhouse, and install heating equipment, humidification equipment and irrigation equipment in the shed , and build a planting frame in the greenhouse at the same time;

[0049] 2. Preparation of cultivation substrate: the cultivation substrate is prepared by mixing sterilized soil, coconut peat, river sand and medicinal dregs according to the ratio of 10:1:2:0.2 in mass ratio, and the medicinal residues are aloe, garlic, mulberry , honeysuckle, Huoxiang extract medicine dregs, the sterilized soil is prepared by mixing poultry manure, humus and quicklime according to the ratio of 3:3:0.1 in mass r...