Pseudomonas fluorescens fermentation medium, culture method and application

A fermentation medium and a technology for Pseudomonas fluorescens, which is applied in the field of microorganisms, can solve the problems that the production capacity of Pseudomonas fluorescens needs to be further improved, and achieve the effects of low cost, increased cell activity, and simple culture and fermentation process.
CN111996136AInactive Publication Date: 2020-11-27PEKING UNIV FOUNDER GRP CO LTD +2
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
PEKING UNIV FOUNDER GRP CO LTD
Publication Date
2020-11-27
Estimated Expiration
Not applicable ยท inactive patent
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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a pseudomonas fluorescens fermentation medium, a culture method and application. The pseudomonas fluorescensfermentation medium contains urea and sodium molybdate, and the mass ratio of the sodium molybdate to the urea is (1-5):(10-30). In addition, glucose, glycerin and soybean oil are used as carbon sources, corn steep liquor is used as a nitrogen source, and sodium chloride, leucine, calcium carbonate and a defoaming agent are included. When the culture medium is used for fermentation culture of pseudomonas fluorescens, the cell activity of pseudomonas fluorescens can be remarkably improved, and the fermentation yield of mupirocin is remarkably increased. In addition, the culture method is low incost, simple and convenient in culture and fermentation process, suitable for popularization and application and suitable for industrial production of mupirocin.
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Description

technical field

[0001] The invention relates to the technical field of microorganisms, in particular to a Pseudomonas fluorescens fermentation medium, a culture method and an application. Background technique

[0002] Mupirocin is produced by fermentation of Pseudomonas fluorescens. Its mechanism of action is to inhibit the first step aminoacylation reaction of bacterial isoleucine-transfer ribonucleic acid synthetase, that is, to inhibit the biosynthesis of isoleucyl adenosine, resulting in the loss of transfer ribonucleic acid.

[0003] Mupirocin produces good antibacterial activity on aerobic Gram-positive cocci, especially Staphylococcus epidermidis, Staphylococcus aureus, and Streptococcus pyogenes, which can infect the skin, and against some Gram-negative bacteria such as influenza Haemophilus, Neisseria gonorrhoeae also have growth inhibitory effect. The scope of mupirocinโ€™s clinical application is gradually expanding, and its side effects are relatively small, and ...

Claims

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