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Pseudomonas fluorescens fermentation medium, culture method and application

A fermentation medium and a technology for Pseudomonas fluorescens, which is applied in the field of microorganisms, can solve the problems that the production capacity of Pseudomonas fluorescens needs to be further improved, and achieve the effects of low cost, increased cell activity, and simple culture and fermentation process.

Inactive Publication Date: 2020-11-27
PEKING UNIV FOUNDER GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the production method of mupirocin is mainly a biological fermentation method, but the production capacity of Pseudomonas fluorescens still needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment provides a Pseudomonas fluorescens fermentation medium of the present invention and a method for producing mupirocin by culturing and fermenting it.

[0037] The raw material formula of fermentation medium is composed as follows in terms of mass percentage: sodium molybdate 0.02%, urea: 0.15%, glucose: 2%, glycerol: 0.5%, sodium chloride: 0.4%, corn steep liquor: 0.4%, leucine 0.1%, calcium carbonate: 0.3%, soybean oil: 1%, foam enemy: 0.02%, and the balance is water.

[0038] The method for cultivating and fermenting mupirocin production is:

[0039] (1) The mupirocin-producing strain (BNCC231887) was verified by experiments, and the slope was passed through the seed bottle, and cultured for 24 hours. Microscopic examination: rod-shaped, large amount of bacteria, deep staining, no miscellaneous bacteria, pH 7.0, mature Seed solution for the next step of fermentation. The slant medium is nutrient agar medium.

[0040] (2) Add 10L of seed medium into a...

Embodiment 2

[0046] This embodiment provides a Pseudomonas fluorescens fermentation medium of the present invention and a method for producing mupirocin by culturing and fermenting it.

[0047] The raw material formula of fermentation medium is composed as follows in terms of mass percentage: sodium molybdate 0.02%, urea 0.15%, glucose: 2%, glycerol: 0.5%, sodium chloride: 0.4%, corn steep liquor: 0.4%, leucine 0.1% %, manganese carbonate: 0.3%, soybean oil: 1%, foam enemy: 0.02%, and the balance is water.

[0048] The method for cultivating and fermenting mupirocin production is:

[0049] (1) The mupirocin-producing strain (BNCC231887) was verified by experiments, and the slope was passed through the seed bottle, and cultured for 24 hours. Microscopic examination: rod-shaped, large amount of bacteria, deep staining, no miscellaneous bacteria, pH 7.0, mature Seed solution for the next step of fermentation. The slant medium is nutrient agar medium.

[0050] (2) Add 300L of seed culture m...

Embodiment 3

[0056] This embodiment provides a Pseudomonas fluorescens fermentation medium of the present invention and a method for producing mupirocin by culturing and fermenting it.

[0057] The raw material formula of specific fermentation medium is composed as follows in mass percent: sodium molybdate 0.01%, urea 0.1%, glucose: 1.5%, glycerol: 0.4%, sodium chloride: 0.3%, corn steep liquor: 0.3%, leucine 0.08%, calcium carbonate: 0.25%, soybean oil: 0.8%, foam enemy: 0.015%, and the balance is water.

[0058] The method for cultivating and fermenting to produce mupirocin is the same as in Example 1, the difference is only in:

[0059] The raw material formula of seed medium is composed as follows in terms of mass percentage: glucose: 1.5%, glycerol: 0.4%, sodium chloride: 0.045%, potassium chloride: 0.045%, calcium carbonate: 0.3%, magnesium sulfate: 0.035%, soybean oil : 0.15%, the rest is water. The pH of the seed medium after sterilization: 6.0.

[0060] The above-mentioned ferm...

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a pseudomonas fluorescens fermentation medium, a culture method and application. The pseudomonas fluorescensfermentation medium contains urea and sodium molybdate, and the mass ratio of the sodium molybdate to the urea is (1-5):(10-30). In addition, glucose, glycerin and soybean oil are used as carbon sources, corn steep liquor is used as a nitrogen source, and sodium chloride, leucine, calcium carbonate and a defoaming agent are included. When the culture medium is used for fermentation culture of pseudomonas fluorescens, the cell activity of pseudomonas fluorescens can be remarkably improved, and the fermentation yield of mupirocin is remarkably increased. In addition, the culture method is low incost, simple and convenient in culture and fermentation process, suitable for popularization and application and suitable for industrial production of mupirocin.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Pseudomonas fluorescens fermentation medium, a culture method and an application. Background technique [0002] Mupirocin is produced by fermentation of Pseudomonas fluorescens. Its mechanism of action is to inhibit the first step aminoacylation reaction of bacterial isoleucine-transfer ribonucleic acid synthetase, that is, to inhibit the biosynthesis of isoleucyl adenosine, resulting in the loss of transfer ribonucleic acid. [0003] Mupirocin produces good antibacterial activity on aerobic Gram-positive cocci, especially Staphylococcus epidermidis, Staphylococcus aureus, and Streptococcus pyogenes, which can infect the skin, and against some Gram-negative bacteria such as influenza Haemophilus, Neisseria gonorrhoeae also have growth inhibitory effect. The scope of mupirocin’s clinical application is gradually expanding, and its side effects are relatively small, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12P17/16C12R1/39
CPCC12N1/20C12N1/38C12P17/162
Inventor 葛艳霞张葵
Owner PEKING UNIV FOUNDER GRP CO LTD
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