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Adeno-associated virus mediated novel coronavirus antibody inducer and vaccine composition

A vaccine composition, coronavirus technology, applied in the field of new coronavirus antibody inducers, to achieve a mild immune response effect

Active Publication Date: 2020-11-27
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on effective novel coronavirus vaccines based on recombinant adeno-associated virus and their mechanisms of action

Method used

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  • Adeno-associated virus mediated novel coronavirus antibody inducer and vaccine composition
  • Adeno-associated virus mediated novel coronavirus antibody inducer and vaccine composition
  • Adeno-associated virus mediated novel coronavirus antibody inducer and vaccine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The mouse novel coronavirus antibody that the antibody inducer of embodiment 1 different immunization antigen induces produces

[0059] 1. AAV viral vector.

[0060] Preparation of AAV virus vector: The sequences shown in SEQ ID No.1, SEQ ID No.4, SEQ ID No.6, and SEQ ID No.8, which were commissioned by GenScript, were digested with BamH1 and EcoRV And connect with the AAV vector sequence (AAV2 / 9 serotype) shown in SEQ ID No.3, obtain the adeno-associated virus based on RBD protein, the adeno-associated virus based on spike protein 1 respectively, based on RBD long protein (abbreviated as L - RBD) adeno-associated virus.

[0061] The packaging of each AAV virus vector was carried out using the method of purification from 293T cell culture medium. When culturing 293T cells (available from conventional reagent companies) in 10 mL of DMEM containing 10% FBS in a 10 cm adsorption dish to a cell density of 70%, use 5 mL of DMEM containing 4% FBS After culturing for 6 hour...

Embodiment 2

[0074] Example 2 Production of New Coronavirus Antibody in Rhesus Monkeys Induced by RBD-Based Antibody Inducers

[0075] 1. Packaging of AAV viral vectors.

[0076] The RBD protein-based adeno-associated virus prepared in Example 1 was used in the immunization experiment on macaques, and the packaging process of the AAV virus vector was as described in Example 1. See the specific plasmid map figure 1 .

[0077] 2. Intramuscular injection of antibody inducer.

[0078] Three rhesus monkeys (provided by Hefei Institute of Physical Science, Chinese Academy of Sciences) were used in the experiment, numbered 4, 5, and 6 respectively. Rhesus monkey No. 4 is male, 6 years old, and weighs 5.6Kg. Macaque No. 5 is a male, 6 years old, weighing 5.8Kg. Rhesus monkey No. 6 is female, 4 years old, and weighs 3.6Kg. Take one cage and raise them. During the whole experimental process up to blood collection, the animals in each group were in good spirits, with normal food intake, no si...

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Abstract

The invention provides a novel coronavirus antibody inducer based on an adeno-associated virus. Antibodies in accordance with a novel coronavirus can be generated in bodies through induction, and theinvention provides a virus vector and a vaccine composition including the antibody inducer.

Description

technical field [0001] The present invention relates to a novel coronavirus antibody inducer prepared by using viral vectors such as adeno-associated virus, which can express a partial antigenic sequence of the novel coronavirus in mammals, thereby enabling organisms to produce antibodies against the novel coronavirus. Background technique [0002] The organism will produce an immune response to foreign substances, such as viruses, proteins, etc., thereby producing antibodies against specific protein sequences or partial sequences, and the antibodies will further bind to the antigenic substances and settle down to be metabolically consumed, protecting the body from the damage of such substances to the body . Using this principle, various inactivated viruses or parts of viruses can be used to prepare vaccines. [0003] At present, the main types of vaccines include: inactivated virus vaccines, recombinant protein vaccines, RNA vaccines, adenovirus vaccines, etc. [0004] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/864C12N15/50A61K39/215A61K39/39A61P31/14
CPCC12N15/86C07K14/005A61K39/12A61K39/39A61P31/14C12N2770/20022C12N2770/20034C12N2750/14143A61K2039/5256
Inventor 薛天金腾川才源章梅童大力
Owner UNIV OF SCI & TECH OF CHINA
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