Kit for detecting coxsackie group B virus IgM antibody by magnetic particle chemiluminiscence method and preparation method of kit

A reagent kit and magnetic particle technology, applied in biochemical equipment and methods, chemical equipment and methods, botany equipment and methods, etc., can solve problems such as narrow linear range, low sensitivity, false positives, etc., to improve linear range, The effect of improving sensitivity and improving specificity

Inactive Publication Date: 2020-11-27
BEIJING BEIER BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The colloidal gold method is generally operated manually, and finally qualitatively judged by the naked eye. This technique has low sensitivity, is prone to false positives, and is greatly affected by human subjective factors. The sensitivity of the enzyme-linked immunosorbent assay is improved compared with the colloidal gold method, but It is still low, and the linear range is narrow, and the response time is also long, which still cannot meet the clinical application well

Method used

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  • Kit for detecting coxsackie group B virus IgM antibody by magnetic particle chemiluminiscence method and preparation method of kit
  • Kit for detecting coxsackie group B virus IgM antibody by magnetic particle chemiluminiscence method and preparation method of kit
  • Kit for detecting coxsackie group B virus IgM antibody by magnetic particle chemiluminiscence method and preparation method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] 1. A test kit for detecting Coxsackie group B virus IgM antibody by magnetic particle chemiluminescence method, said test kit comprising:

[0071] Enzyme label working solution, magnetic bead suspension, sample diluent, calibrator and luminescent substrate solution;

[0072] Wherein, the enzyme-labeled working solution includes the following final concentration components: bovine serum albumin (BSA) 2.5g / L, newborn calf serum 100mL / L, sodium chloride 8.0g / L, disodium hydrogen phosphate 5.8g / L , sodium dihydrogen phosphate 0.593g / L and horseradish peroxidase-labeled Coxsackie group B virus antigen 1 μg / mL;

[0073] The magnetic bead suspension includes the following final concentration components: 1 mg / mL of magnetic particles coupled with mouse anti-human IgM antibody (based on the mass of magnetic particles), wherein 1 mg of magnetic particles is coupled with 40 μg of mouse anti-human IgM antibody; the magnetic particles are superparamagnetic particles; each 100mL mag...

Embodiment 2

[0093] 1. A magnetic particle chemiluminescence method detects a test kit for Coxsackie group B virus IgM antibody, the test kit is the same as Example 1, the difference is:

[0094] The Coxsackie B group virus antigen is recombinant antigen B.

[0095] 2. The preparation method of the kit is the same as in Example 1.

Embodiment 3

[0097] 1. A magnetic particle chemiluminescence method detects a test kit for Coxsackie group B virus IgM antibody, the test kit is the same as Example 1, the difference is:

[0098] The Coxsackie B group virus antigen is recombinant antigen C.

[0099] 2. The preparation method of the kit is the same as in Example 1.

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Abstract

The embodiment of the invention relates to the field of immunodetection, in particular to a kit for detecting a coxsackie group B virus IgM antibody by a magnetic particle chemiluminiscence method anda preparation method of the kit. The kit comprises an enzyme-labeled working solution, a magnetic bead suspension, a sample diluent, a calibrator and a luminous substrate solution, wherein the enzyme-labeled working solution comprises a horse radish peroxidase labeled coxsackie group B virus antigen, and the magnetic bead suspension comprises magnetic particles coupled with a mouse anti-human IgMantibody; the coxsackie group B virus antigen comprises one or more of a recombinant antigen A, a recombinant antigen B or a recombinant antigen C; the amino acid sequence of the recombinant antigenA comprises a sequence as shown in SEQ ID NO.1; the amino acid sequence of the recombinant antigen B comprises a sequence as shown in SEQ ID NO.2; and the amino acid sequence of the recombinant antigen C comprises a sequence shown as SEQ ID NO. 3. According to the kit provided by the embodiment of the invention, the sensitivity and the linear range are improved, and the specificity and the accuracy are high.

Description

technical field [0001] The invention relates to the field of immunodetection, in particular to a kit for detecting IgM antibody of Coxsackie B group virus by magnetic particle chemiluminescence method and a preparation method thereof. Background technique [0002] Coxsackie virus is the causative agent of very serious human diseases. In 1948, Dolldorf and Sickles isolated a group of viruses from stool samples of patients clinically diagnosed as polio in Coxsackie Township, New York, USA, belonging to small RNA viruses. Can cause many diseases ranging from upper respiratory tract to central nervous system infection. The virus is divided into two groups, A and B, among which the Coxsackievirus group B (CVB) virus belongs to the Enterovirus genus of the family Picornaviridae, has 6 serotypes, and is the cause of human viral myocarditis, aseptic It is the main pathogen of diseases such as meningitis and neonatal systemic infection, which can spread or break out all over the wor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/535G01N21/76C07K14/085C12N15/41
CPCG01N33/56983G01N33/54326G01N33/535G01N21/76C07K14/005G01N2469/20G01N2333/085C12N2770/32022
Inventor 徐悦
Owner BEIJING BEIER BIOENG
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