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Method and apparatus for detecting a plurality of target nucleic acid sequences in sample

A target nucleic acid sequence and detection sample technology, applied in biochemical equipment and methods, microbial measurement/inspection, instruments, etc., can solve the problem of consuming a lot of time for unzipping analysis, and achieve the effect of reducing noise signal and signal deviation

Active Publication Date: 2020-11-27
SEEGENE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Melting analysis in each cycle consumes a lot of time

Method used

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  • Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
  • Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
  • Method and apparatus for detecting a plurality of target nucleic acid sequences in sample

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Embodiment approach

[0445] The present invention will be described in more detail through examples. These examples are for more specific illustrations, and it is obvious to those skilled in the art that the scope of the present invention is not limited to the examples.

Embodiment 1

[0447] Embodiment 1: the detection of target nucleic acid sequence (single target)

[0448] Example 1 exemplifies the prediction T of dimers associated with target nucleic acid sequences m An example of the present invention in which two detected temperatures are determined based on the values ​​and used as reference temperatures.

[0449] Preparation of Signal Generating Compositions

[0450] In order to detect Chlamydia trachomatis L (Chlamydia trachomatis serovars L (CT)), 4 samples were mixed with Allplex TM The signal generating composition (ie, oligonucleotide mix and enzyme master mix) contained in the STI-GU kit (Seegene, Inc., catalog number: SD9802X) was mixed.

[0451] Signal generating compositions include compositions for forming dimers that provide a detectable signal associated with a target nucleic acid sequence by detection and labeling oligonucleotide cleavage and extension methods. In the presence of a target nucleic acid sequence in the sample, the medi...

Embodiment 2

[0469] Example 2: Detection of Target Nucleic Acid Sequences (Multiple Targets)

[0470] Example 2 exemplifies the predicted T of dimers associated with the target nucleic acid sequence m An example of the present invention in which two detected temperatures are determined on the basis of the values ​​and used as reference temperatures.

[0471] Preparation of Signal Generating Compositions

[0472] In order to detect Chlamydia trachomatis L and Treponema pallidum (TP), 4 samples were mixed with Allplex TM The signal generating composition contained in the STI-GU kit (Seegene, Inc., catalog number: SD9802X) was mixed.

[0473] In the presence of CT, the predicted T of the extended dimer m It is 64.5°C. The template site of the capture and template oligonucleotides associated with CT has a dual label of Cal Red 610 and BHQ2. In the presence of TP, the predicted T of the extended dimer m It is 76°C. The template site of the TP-associated capture and template oligonucleot...

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Abstract

The method of the present invention enables efficient detection of a plurality of target nucleic acid sequences in one detection channel, by obtaining a data set of cycle / signal-change value.

Description

[0001] Cross References to Related Applications [0002] This application claims priority from Korean Patent Application No. 2018-0046375 filed on April 20, 2018, the entire contents of which are incorporated herein by reference. technical field [0003] The present invention relates to the detection of multiple target nucleic acid sequences in a sample using signal change values. Background technique [0004] Real-time polymerase chain reaction (real-time PCR) is a polymerase chain reaction-based technology (Logan J et al., (2009). Real-Time PCR: Current Technology and Applications. Caister for real-time detection of target nucleic acids in samples Academic Press). To detect a target nucleic acid molecule, real-time polymerase chain reaction uses a signal generating composition that generates a detectable fluorescent signal in proportion to the amount of the target molecule. Generation of a fluorescent signal can be achieved by using intercalators that generate a signal w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6823G16B40/10
CPCG16B40/10C12Q1/6816C12Q2525/161C12Q2527/107C12Q2537/143C12Q2565/1015C12Q2527/101C12Q2565/537C12Q2537/165C12Q1/6823
Inventor 高成文韩知慧金英旭朴永用
Owner SEEGENE INC