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Application of pseudomonas rhodesiae in restoration of heavy metal polluted environment and heavy metal tolerance

A technology for Pseudomonas Roche and environmental pollution, applied in the field of microorganisms, can solve problems such as unfavorable rapid repair, large amount of engineering, secondary pollution, etc.

Inactive Publication Date: 2020-12-01
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical and chemical repair methods have high repair efficiency and fast speed, but the amount of work is large, the cost is high, and it is easy to cause secondary pollution
Compared with them, the cost of bioremediation is lower and it is not easy to cause secondary pollution, but the repair cycle of phytoremediation is long, which is not conducive to the purpose of rapid repair

Method used

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  • Application of pseudomonas rhodesiae in restoration of heavy metal polluted environment and heavy metal tolerance
  • Application of pseudomonas rhodesiae in restoration of heavy metal polluted environment and heavy metal tolerance
  • Application of pseudomonas rhodesiae in restoration of heavy metal polluted environment and heavy metal tolerance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Pseudomonas roseri to the solubility test of insoluble phosphorus

[0043] Strain: Pseudomonas rhodesiae can be purchased from ATCC 700311 TM .

[0044] Take Pseudomonas rosenbergii out of the -80°C refrigerator for activation, inoculate it in beef extract-peptone liquid medium, cultivate it in a shaker at 25-30°C, 140rpm for 24 hours, and inoculate it with 1% inoculum In the fresh beef extract-peptone liquid medium, after culturing under the same culture conditions for 24 hours, the culture solution was centrifuged under sterile conditions, the supernatant was poured off, the cells were washed 3 times with 0.9% NaCl, and resuspended in 0.9% NaCl solution to obtain a Pseudomonas roserii bacteria liquid, which was stored at 4°C until use. The purpose of washing with 0.9% NaCl is to wash away the complex components in the beef extract-peptone medium, so as to reduce the interference on the results of subsequent phosphate solubilization experiments. In the act...

Embodiment 2

[0049] The pH change of NBRIP liquid medium after the embodiment 2 inoculates Pseudomonas roseri

[0050] This example is used to illustrate the change of pH value of NBRIP liquid medium after inoculating Pseudomonas roseri.

[0051] Inoculate the Pseudomonas roserii bacteria liquid obtained above in the NBRIP liquid medium with an inoculum amount of 1%. On days 0, 1, 3, 5, and 9, take 10ml of the suspension respectively, and use a pH meter to measure the respective pH value.

[0052] Set up a comparative example: according to the above method, measure the change of the pH value of the NBRIP liquid medium under the natural state, that is, without adding any bacterial agent. The difference: do not add any bacteria agent.

[0053] The result is as Figure 4 shown. From Figure 4 It can be seen from the figure that after inoculation of the Pseudomonas roserii bacteria solution, the pH of the culture solution immediately decreased from 6.3 to below 4.2, and remained below 4.3...

Embodiment 3

[0055] Example 3 Pseudomonas roseri on Pb 2+ Solubility Test of Insoluble Phosphorus under Stress

[0056] This example is used to illustrate the presence of Pseudomonas roseri on Pb 2+ The ability to dissolve insoluble phosphorus under stress.

[0057] The above-mentioned obtained Pseudomonas roserii bacterium liquid was inoculated with 1% inoculum amount in containing respectively 0,100,200,300,400,600mg / L Pb 2+ NBRIP liquid medium. On the 0th, 1st, 3rd, 5th, and 9th days, 10ml of the suspension was taken respectively, and after centrifugation at a speed of 6000rpm / min for 10min in a centrifuge, the content of dissolved P in the supernatant was measured using an inductively coupled plasma spectrometer (ICP-OES) .

[0058] The result is as Figure 5 shown. From Figure 5 It can be seen that with the Pb 2+ As the concentration increases, the content of soluble P decreases, because the dissolved P is used for Pb 2+ Fixed and consumed. Even at 600mg / L Pb 2+ Under stre...

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Abstract

The invention discloses an application of pseudomonas rhodesiae in restoration of heavy metal polluted environment and heavy metal tolerance. The pseudomonas rhodesiae can restore lead-polluted soil or water. Indissolvable phosphorus or dissolved phosphorus can be dissolved, and lead is converted into stable phosphorus-chlorine-lead ore.

Description

technical field [0001] The invention relates to the field of microbes, in particular to the use of Pseudomonas roseri for repairing heavy metal polluted environments and heavy metal tolerance. Background technique [0002] With the rapid development of economy and society, while bringing great convenience to people's life, it also brings great pollution to the environment. In recent years, soil heavy metal pollution has become more and more serious. According to the "National Soil Pollution Survey Bulletin" issued by the Ministry of Environmental Protection and the Ministry of Land and Resources in 2014, about one-fifth of the soil is polluted by heavy metals to varying degrees, and the annual loss of grain reaches tens of millions of tons. , causing serious harm to human health. In recent years, there have been many lead poisoning incidents in many places in my country, especially the concentrated occurrence of a series of incidents of excessive lead content in children's...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/002B09C1/105B09C2101/00
Inventor 赵峰李俊鹏
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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