Spinal cord injury rat primary microglial cell separation method and application

Abstract

The invention discloses a spinal cord injury rat primary microglial cell separation method and application. The method takes rats with spinal cord injury in a short period as a test raw material, andconsiderable quantities of high-purity microglial cells can be quickly obtained through simple grinding, filtering and other steps; according to the method, the time for extracting the microglial cells by a traditional method is greatly shortened, and a basis is provided for dynamically detecting the functions of the in-vivo microglial cells in real time. The method overcomes the defects of difficult microglial cell purification and high process cost of traditional methods, can acquire high-purity microglial cells efficiently and economically, and provides a basis for research of microglial cells after spinal cord injury.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to the preparation of biological models, the separation, identification and culture technology of functional cells, in particular to the separation method and application of primary microglial cells in rats with spinal cord injury. Background technique [0002] Spinal cord injury can lead to the loss of sensory and motor functions, and is one of the diseases that seriously damage the central nervous system, which brings heavy economic burden to society and family. Microglia are the innate immune cells of the central nervous system, which are activated in the early stage of spinal cord injury, not only participate in the maturation of the central nervous system, but also directly affect the neuron regeneration in adulthood. For example, in the injured optic nerve or spinal cord, implanting new microglia or macrophages can significantly improve the post-traumatic regeneration of ner...

AI Technical Summary

Problems solved by technology

[0003] At present, there have been a large number of reports on the culture method of neonatal mouse cortex microglia, mainly by culturing mixed glial cells, and then adopting "gentle trypsinization method", "layer shaking method", "density gradient centrifugation method" or " However, there are few literature reports on the isolation and purification methods of spinal cord microglial cells, and most of them refer to the above culture methods. The cycle is about 10 days longer, and the yield and purity are not high. high

In addition, there are relatively few microglia extraction methods after spinal cord injury, and the traditional microglia extraction methods are mostly cells that have been cultured and proliferated for a period of time (the state has changed), and a large number cannot be obtained in a short period of time. The high purity of microglia, which is quite different from that of somatic cells, restricts the research on the mechanism of microglia after spinal cord injury

Images