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Therapeutic agent for spinal cord injury

A technique for spinal cord injury and spinal cord, applied in the field of cell preparation of pluripotent stem cells, to achieve the effect of improving or restoring motor function

Pending Publication Date: 2020-12-11
LIFE SCI LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no examples of using Muse cells to treat spinal cord injuries and showing the desired therapeutic effect

Method used

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  • Therapeutic agent for spinal cord injury
  • Therapeutic agent for spinal cord injury
  • Therapeutic agent for spinal cord injury

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0081] (2) Preparation and use of cell preparations

[0082] The cell preparation of the present invention is not particularly limited, and can be obtained by suspending the Muse cells obtained in (1) above or a cell population containing Muse cells in physiological saline or an appropriate buffer (for example, phosphate-buffered saline). In this case, if the number of Muse cells isolated from autologous or allogeneic tissues is small, they can be cultured before cell transplantation until they proliferate to obtain a predetermined number of cells. In addition, as already reported (International Publication No. WO 2011 / 007900 pamphlet), Muse cells do not become tumors, so even if the cells recovered from living tissue contain an undifferentiated state, the possibility of their canceration is low. It's low, so it's safe. In addition, culture of the recovered Muse cells is not particularly limited, and can be performed in an ordinary growth medium (for example, α-minimal essent...

Embodiment 1-1

[0093] Example 1-1: Preparation of Rat Spinal Cord Injury Model

[0094] Vertebrectomy was performed on the 9th to 10th thoracic vertebrae of 11-week-old female SD rats under anesthesia to expose the spinal cord, and a weight (diameter 2.5 mm, weighing 10 grams) dropped from a height of 25mm to injure the spinal cord (3 groups, 8 rats in each group).

Embodiment 1-2

[0095] Example 1-2: Preparation of human Muse cells

[0096] Muse cells were obtained according to the method described in International Publication No. WO 2011 / 007900 concerning the isolation and identification of human Muse cells. More specifically, Muse cells are obtained by expanding enrichment culture by culturing mesenchymal stem cells under stress conditions.

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PUM

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Abstract

Provided is a cell preparation for treating a spinal cord injury, the cell preparation including pluripotent stem cells (Muse cells) which are SSEA-3 positive and derived from mesenchymal tissues of aliving body or cultured mesenchymal cells. The spinal cord injury preferably includes a complete spinal cord injury or an incomplete spinal cord injury.

Description

【Technical field】 [0001] The present invention relates to cell preparations in regenerative medicine. More specifically, the present invention relates to cell preparations containing pluripotent stem cells effective in the treatment of spinal cord injuries. 【Background technique】 [0002] Spinal cord injury (Spinal Cord Injury) mainly refers to the state in which a strong external force acts on the spine and damages the spine and the spinal cord. In addition, similar symptoms are also produced due to internal causes such as spinal cord tumors and hernias (hereinafter, these may be collectively referred to as spinal cord injuries). [0003] At present, it is said that there are more than 100,000 spinal cord injury patients in Japan, and more than 5,000 people have new spinal cord injuries every year. The causes of injury in descending order of proportion are: 1. Traffic accident, 2. Fall from height, 3. Fall, 4. Collision / crushing injury, 5. Sports, 6. Others, etc. [0004...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/545A61K35/28A61P7/00A61P25/00C12N5/0775
CPCA61P25/00A61K35/28A61P21/00A61P7/00
Inventor 川村诚桝富直哉
Owner LIFE SCI LAB