HPLC specific chromatogram of ampelopsis japonica formula granules as well as construction method and application thereof

A technology of formula granules and characteristic map, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of undocumented quality control methods of radix radix formula granules, and achieve the effect of complete chromatographic peak information and uniform chromatographic peak distribution.

Inactive Publication Date: 2020-12-15
SICHUAN NEO GREEN PHARMA TECH DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no record about any quality control method of Baikang formula granules. For this reason, the present invention provides a HPLC characteristic spectrum of Baikous formula granules and its construction method and application

Method used

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  • HPLC specific chromatogram of ampelopsis japonica formula granules as well as construction method and application thereof
  • HPLC specific chromatogram of ampelopsis japonica formula granules as well as construction method and application thereof
  • HPLC specific chromatogram of ampelopsis japonica formula granules as well as construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] This example relates to the HPLC characteristic spectrum and its construction method of the white pomegranate formula granules.

[0061] Preparation of the test solution: Take an appropriate amount of white scorpion formula granules, grind it finely, take about 0.5g, weigh it accurately, add 20ml of 50% methanol, ultrasonicate (power 600W, frequency 40kHz) for 30min, take it out, let cool to 25°C, Shake well, filter, and take the continued filtrate to obtain the test solution of the white pomegranate formula granules.

[0062] Preparation of reference substance solution: Take 1.0g of the reference medicinal material of Radix Sinensis, put it in a conical flask, add 25ml of 50% methanol, ultrasonicate (power 600W, frequency 40kHz) for 30min, take it out, let it cool to 25°C, shake well, filter, Take the continued filtrate to obtain the reference substance solution of the Radix Sinensis reference medicinal material.

[0063] Prepare the reference substance solution of th...

Embodiment 2

[0076] The construction method described in Example 1 is used to construct the HPLC characteristic collection of basalt formula granules, the difference is only that the specific parameters for preparing the test solution and the preparation of the reference solution are different, as follows:

[0077] Preparation of the test solution: Take an appropriate amount of white scorpion formula granules, grind it finely, take about 0.5g, weigh it accurately, add 20ml of 50% methanol, ultrasonicate (power 600W, frequency 40kHz) for 20min, take it out, let cool to 15°C, Shake well, filter, and take the continued filtrate to obtain the test solution of the white pomegranate formula granules.

[0078] Preparation of reference substance solution: take 1.0g of the reference medicinal material of Radix Sinensis, put it in a conical flask, add 25ml of 50% methanol, ultrasonicate (power 600W, frequency 40kHz) for 20min, take it out, let cool to 15°C, shake well, filter, Take the continued fil...

Embodiment 3

[0080] The construction method described in Example 1 is used to construct the HPLC characteristic collection of basalt formula granules, the difference is only that the specific parameters for preparing the test solution and the preparation of the reference solution are different, as follows:

[0081] Preparation of the test solution: take an appropriate amount of white scorpion formula granules, grind finely, take about 0.5g, accurately weigh, add 20ml of 50% methanol, ultrasonically treat (power 600W, frequency 40kHz) for 40min, take it out, let cool to 35°C, Shake well, filter, and take the continued filtrate to obtain the test solution of the white pomegranate formula granules.

[0082] Preparation of reference substance solution: Take 1.0g of the reference medicinal material of Radix Sinensis, put it in a conical flask, add 25ml of 50% methanol, ultrasonicate (power 600W, frequency 40kHz) for 40min, take it out, let cool to 35°C, shake well, filter, Take the continued fi...

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Abstract

The invention discloses an HPLC specific chromatogram of ampelopsis japonica formula granules as well as a construction method and application thereof, and provides a comprehensive, rapid and effective internal quality detection method for the ampelopsis japonica formula granules through construction and use of the HPLC specific chromatogram, and the characteristics of simplicity in operation, stability, reliability and high precision are achieved.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicinal materials, in particular, it relates to an HPLC characteristic spectrum of white pomegranate formula granules, a construction method and application thereof. Background technique [0002] Traditional Chinese medicine formula granules are granules made by extracting, separating, concentrating, drying, and granulating single-flavored Chinese medicine decoction pieces. They lose the morphological characteristics of the original decoction pieces. The authenticity identification and evaluation of their pros and cons have always restricted the development of the industry. bottleneck. Therefore, it is one of the key issues that need to be urgently solved at present to establish a quality evaluation system for traditional Chinese medicine formula granules with associated efficacy and fingerprint characteristics to fully reflect and effectively control the intrinsic quali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8675
Inventor 周厚成胡昌江姚丽琴罗俊李莎冯健周维
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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