Characteristic chromatogram based on fructus gleditsiae extract, and construction method and application thereof

A feature map and construction method technology, applied in the field of traditional Chinese medicine detection, can solve the problems of single component detection and incomplete control, and achieve the effects of ensuring the quality of finished medicines, rapid quality identification, and simple operation

Pending Publication Date: 2020-03-27
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on the above situation, it can be known that the content determination of active substances in pig tooth soap can only realize the detection of a single component at present, and the control is not comprehensive. We know that the result of single component detection cannot reflect the internal quality of pig tooth soap as a whole. Therefore, It is especially important to establish a method for comprehensive, rapid and objective evaluation of its intrinsic quality

Method used

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  • Characteristic chromatogram based on fructus gleditsiae extract, and construction method and application thereof
  • Characteristic chromatogram based on fructus gleditsiae extract, and construction method and application thereof
  • Characteristic chromatogram based on fructus gleditsiae extract, and construction method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1: need testing product solution extraction solvent investigation.

[0074] According to the determined chromatographic conditions, the extraction method of ultrasonic treatment was adopted, and water, 50% methanol and methanol were used as extraction solvents respectively to investigate the pretreatment method of the sample extract powder; 50% methanol was used as the extraction solvent, filtered, and the filtrate was evaporated to dryness, and the residue was dissolved with 50% methanol, and the pretreatment method of the sample decoction powder was investigated. figure 1 , figure 1 For the determination of the preparation method of the test sample of pig tooth soap, extract powder water, extract powder 50% methanol, extract powder methanol, decoction piece water, decoction piece 50% methanol are used sequentially from bottom to top in the figure.

[0075] Specifically as follows:

[0076] Take 0.3 g of Zhuyazao 191-2-6 extract powder, accurately weigh th...

Embodiment 2

[0079] Embodiment 2: Screening of chromatographic conditions.

[0080] Consult the relevant information of pig tooth soap, and preliminarily draw up the liquid phase detection method of the characteristic map of pig tooth soap as follows:

[0081] Diode array detector, wavelength range 190-600 nm; Welch Ultimate® XB-C18 column (4.6 mm×250 mm×5 μm); column temperature 30 ℃; flow rate 1.0 ml / min; acetonitrile as mobile phase A, Water was used as mobile phase B for gradient elution, and the gradient elution conditions were shown in the table below (Table 1).

[0082] Table 1 Preliminary test gradient elution table

[0083]

[0084] Take 0.3 g of pig tooth soap (191-2-6) extract powder, accurately weigh it, put it in a stoppered conical flask, add 10 ml of 50% (ml / ml) methanol precisely, seal it tightly, weigh it, and ultrasonically Treat for 30 min, let it cool to room temperature, weigh again, make up the lost weight with 50% methanol, shake well, filter, and take the filtr...

Embodiment 3

[0094] Embodiment 3: the detection of the characteristic spectrum of pig tooth soap extract.

[0095] According to the method determined, prepare the need testing solution of 15 batches of standard decoctions, sample injection detects respectively, the result is as follows: Figure 7 As shown, the order from bottom to top in the figure is: 1→15. The results showed that the spectral results of 15 batches of standard decoctions made from different sources of decoction pieces were highly similar, and each characteristic peak appeared.

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Abstract

The invention discloses a characteristic chromatogram based on a fructus gleditsiae extract, and a construction method and application thereof, provides a comprehensive, rapid and effective internal quality detection method for the fructus gleditsiae extract through construction and use of the characteristic chromatogram, and has the advantages of simplicity in operation, stability, reliability and high precision.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and specifically relates to a characteristic map based on pig tooth soap extract and its construction method and application. Background technique [0002] Pig tooth soap is the dry sterile fruit of Gleditsia sinensis Lam., a plant of the genus Gleditsia Lann. Spicy, salty, warm, returns to the lung and large intestine meridian, has the effect of dispelling phlegm, resuscitating swelling, dispelling stagnation, and is used for apoplexy, coma, epilepsy, phlegm obstruction, persistent disease, wheezing and coughing, unpleasant expectoration, and dry stool ; External treatment of carbuncle. According to current research reports, pig tooth soap mainly contains a variety of triterpene saponins, in addition, there are tannins, wax alcohol, nonacane, stigmasterol, sitosterol, trace elements, etc. It has pharmacological activities such as anti-inflammatory, anti-allergic,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N30/34G01N2030/047
Inventor 陈雪梅李远志钟钰张佳卉肖斌张巨琼王晨曦陈发贵朱永红
Owner CHENGDU PUSH BIOLOGICAL TECH
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