UPLC specific chromatogram of rabdosia rubescens formula granules, and construction method and application thereof

A technology of formula granules and characteristic maps, which is applied in the field of UPLC characteristic maps and construction of Donglingcao formula granules. evenly distributed effect

Active Publication Date: 2020-12-15
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be known from this that the prior art has disclosed the rapid and accurate determination method of UPLC in the process of establishing the quality control of Rubescensine A in the medicinal materials of Oryxba radix L., but for the formula granules of Rexybes oriolis, there is no decoction piece The characteristics of character identification, therefore, the establishment of UPLC characteristic map has identification significance for Donglingcao formula granules

Method used

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  • UPLC specific chromatogram of rabdosia rubescens formula granules, and construction method and application thereof
  • UPLC specific chromatogram of rabdosia rubescens formula granules, and construction method and application thereof
  • UPLC specific chromatogram of rabdosia rubescens formula granules, and construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] This example relates to a method for constructing a UPLC characteristic map of Oridonium radix formula granules.

[0060] Preparation of the test solution: Take 1.0g of the formula granules of Ornithus fragrans, put it in a stoppered Erlenmeyer flask, add 25ml of 70% methanol, ultrasonicate (power 600W, frequency 40kHz) for 45min, let cool to 25°C, shake well and filter After that, take the continued filtrate, as the test solution of Oridonium formula granules.

[0061] Preparation of reference solution: Take 1.0g of the reference herbal material of Dongling, put it in a conical flask with a stopper, add 25ml of 70% methanol, ultrasonicate (power 600W, frequency 40kHz) for 45min, let cool to 25°C, shake well and filter , and take the continued filtrate as the reference solution for the reference medicinal material of Ornithus radix L.

[0062] Take another appropriate amount of Rubescensin A reference substance, weigh it accurately, add 70% methanol to make a solution ...

Embodiment 2

[0074] The construction method described in Example 1 is used to construct the UPLC characteristic collection of Radix Grass Formula Granules, the difference is only that the specific parameters for preparing the test solution and the preparation of the reference solution are different, as follows:

[0075] Preparation of the test solution: Take 1.0g of the formula granules of Rubescens japonicus, put it into a stoppered Erlenmeyer flask, add 25ml of 70% methanol, ultrasonicate (power 600W, frequency 40kHz) for 30min, let cool to 30°C, shake well and filter After that, take the continued filtrate, as the test solution of Oridonium formula granules.

[0076] Preparation of reference substance solution: Take 1.0g of the reference medicinal material of Oryx nigra, put it in a conical flask with a stopper, add 25ml of 70% methanol, ultrasonicate (power 600W, frequency 40kHz) for 30min, let cool to 30°C, shake well and filter , and take the continued filtrate as the reference solut...

Embodiment 3

[0078] The construction method described in Example 1 is used to construct the UPLC characteristic collection of Radix Grass Formula Granules, the difference is only that the specific parameters for preparing the test solution and the preparation of the reference solution are different, as follows:

[0079] Preparation of the test solution: Take 1.0g of the formula granules of Ornithus fragrans, put it in a stoppered Erlenmeyer flask, add 25ml of 70% methanol, ultrasonicate (power 600W, frequency 40kHz) for 15min, let cool to 20°C, shake well and filter After that, take the continued filtrate, as the test solution of Oridonium formula granules.

[0080] Preparation of reference substance solution: Take 1.0g of the reference medicinal material of Oryx sativa, put it into a stoppered Erlenmeyer flask, add 25ml of 70% methanol, sonicate (power 600W, frequency 40kHz) for 15min, let cool to 20°C, shake well and filter , and take the continued filtrate as the reference solution for ...

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Abstract

The invention provides a UPLC specific chromatogram of rabdosia rubescens formula granules, a method for constructing the UPLC specific chromatogram and application of the UPLC specific chromatogram in oridonin A detection in order to establish a method high in accuracy and precision and capable of rapidly evaluating the internal quality of the rabdosia rubescens formula granules. By constructingand applying theUPLC specific chromatogram, the comprehensive, rapid and effective internal quality detection method is provided for the rabdosia rubescens formula granules, and the method has the characteristics of simple operation, stability, reliability and high precision.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicinal materials, in particular, it relates to a UPLC characteristic spectrum of Donglingcao formula granules and its construction method and application. Background technique [0002] Traditional Chinese medicine formula granules are granules made of single-flavored Chinese medicine decoction pieces through water extraction, separation, concentration, drying, and granulation. They lose the morphological characteristics of the original decoction pieces. The authenticity identification and evaluation of their pros and cons have always restricted the development of the industry. bottleneck. Therefore, it is one of the key issues that need to be urgently solved at present to establish a quality evaluation system for traditional Chinese medicine formula granules with associated efficacy and fingerprint characteristics to fully reflect and effectively control the intrinsic q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8634G01N30/8679
Inventor 周厚成胡昌江李莎郑传奎吴琴周维姚丽琴
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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