A kind of ULC characteristic map of Rubescens rubescens formula granule and its construction method and application

A technology of formula granules and characteristic maps, which is applied in the field of UPLC characteristic maps and its construction of Donglingcao formula granules, can solve the problems of not having the identification characteristics of decoction pieces, so as to ensure the quality of finished medicines, strong specificity, and chromatographic peak information full effect

Active Publication Date: 2022-07-22
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be known from this that the prior art has disclosed the rapid and accurate determination method of UPLC in the process of establishing the quality control of Rubescensine A in the medicinal materials of Oryxba radix L., but for the formula granules of Rexybes oriolis, there is no decoction piece The characteristics of character identification, therefore, the establishment of UPLC characteristic map has identification significance for Donglingcao formula granules

Method used

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  • A kind of ULC characteristic map of Rubescens rubescens formula granule and its construction method and application
  • A kind of ULC characteristic map of Rubescens rubescens formula granule and its construction method and application
  • A kind of ULC characteristic map of Rubescens rubescens formula granule and its construction method and application

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Embodiment 1

[0059] The present embodiment relates to a method for constructing a UPLC characteristic map of Rubescens rubescens formula granules.

[0060] Preparation of test solution: take 1.0 g of Rubescens rubescens formula granules, put in a stoppered conical flask, add 25 ml of 70% methanol, ultrasonically treat (power 600W, frequency 40kHz) for 45min, let cool to 25 ℃, shake well and filter After passing, take the continuous filtrate as the test solution of Rubescensica granules.

[0061] Preparation of reference solution: take 1.0 g of Dongling reference herbal material, put it in a conical flask with stopper, add 25 ml of 70% methanol, ultrasonically treat (power 600W, frequency 40kHz) for 45min, let cool to 25 ℃, shake well and filter , take the subsequent filtrate, as the reference solution of Rubescens' reference medicinal material.

[0062] Another appropriate amount of oridonin A reference substance was taken, accurately weighed, and 70% methanol was added to make a solution...

Embodiment 2

[0074] Adopt the construction method described in Example 1 to construct the UPLC characteristic spectrum of Rubescens rubescens formula granules, and the difference is only in that the specific parameters of preparing the test solution and preparing the reference solution are different, and the details are as follows:

[0075] Preparation of test solution: take 1.0 g of Rubescens rubescens formula granules, put in a stoppered conical flask, add 25 ml of 70% methanol, ultrasonically treat (power 600W, frequency 40kHz) for 30min, let cool to 30 ℃, shake well and filter After passing, take the continuous filtrate as the test solution of Rubescensica granules.

[0076] Preparation of reference substance solution: take 1.0 g of Rubescens' reference medicinal material, put it in a stoppered conical flask, add 25 ml of 70% methanol, ultrasonically treat (power 600W, frequency 40kHz) for 30min, let cool to 30 ℃, shake well and filter , take the subsequent filtrate, as the reference s...

Embodiment 3

[0078] Adopt the construction method described in Example 1 to construct the UPLC characteristic spectrum of Rubescens rubescens formula granules, and the difference is only in that the specific parameters of preparing the test solution and preparing the reference solution are different, and the details are as follows:

[0079] Preparation of test solution: take 1.0 g of Rubescens rubescens formula granules, put in a stoppered conical flask, add 25 ml of 70% methanol, ultrasonically treat (power 600W, frequency 40kHz) for 15min, let cool to 20 ℃, shake well and filter After passing, take the continuous filtrate as the test solution of Rubescensica granules.

[0080] Preparation of reference solution: take 1.0g of Rubescens' reference medicinal material, put it in a stoppered conical flask, add 25ml of 70% methanol, ultrasonically treat (power 600W, frequency 40kHz) for 15min, let cool to 20 ℃, shake well and filter , take the subsequent filtrate, as the reference solution of R...

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Abstract

In order to establish a method of high accuracy and precision and to quickly evaluate the internal quality of Rubescens rubescens formula granules, the invention provides a UPLC characteristic spectrum of Rubescens rubescens formula granules, a method for constructing the UPLC characteristic spectrum and the UPLC characteristic spectrum in The application in the detection of Rubescensine A, through the construction and use of the UPLC characteristic map, provides a comprehensive, rapid and effective method for the detection of the internal quality of Rubescensine Formula Granules, which is easy to operate, stable and reliable, and has high precision. Features.

Description

technical field [0001] The invention belongs to the technical field of detection of Chinese medicinal materials, and in particular relates to a UPLC characteristic map of Rubescens rubescens formula granules and a construction method and application thereof. Background technique [0002] Traditional Chinese medicine formula granules are granules obtained from single-flavored Chinese herbal medicine pieces through water extraction, separation, concentration, drying and granulation. They have lost the morphological characteristics of the original pieces. bottleneck. Therefore, the establishment of a quality evaluation system for traditional Chinese medicine formula granules with associated efficacy and fingerprint characteristics to comprehensively reflect and effectively control the inherent quality of formula granules is one of the key problems that needs to be solved urgently. [0003] Rubus spp., scientific name Broken Rice ( Rabdosia rubescens (Hemsl.) Hara ), is a plan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8634G01N30/8679
Inventor 周厚成胡昌江李莎郑传奎吴琴周维姚丽琴
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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