Kit for combined detection of acute myeloid leukemia

A combined detection technology for acute myeloid cells, applied in the field of molecular biological detection, can solve problems such as increased detection time and complex data analysis process
CN112094914AActive Publication Date: 2020-12-18苏州科贝生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
苏州科贝生物技术有限公司
Publication Date
2020-12-18

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Abstract

The invention provides a kit for combined detection of acute myeloid leukemia. The kit comprises a special linker for DNA library construction, a specific composite primer for detecting gene rearrangement and gene whole exon mutation, and a composite primer for library amplification. The special linker is 8 dimers formed by a linker primer 1 and eight different i5-terminal primers respectively; the types of the gene rearrangement and the gene whole exon mutation are CBFB rearrangement, RUNX1 rearrangement, MLL rearrangement, RARA rearrangement, MECOM rearrangement and TP53 gene whole exon mutation; and the composite primer for library amplification has different i7 terminals. The kit provided by the invention can be used for detecting a plurality of common molecular genetic variations of acute myeloid leukemia at one time, and is simple to operate, short in time and high in detection efficiency.
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Description

technical field

[0001] The invention belongs to the field of molecular biological detection, in particular to a kit for combined detection of acute myeloid leukemia. Background technique

[0002] Acute myeloid leukemia (AML) is a malignant disease of myeloid hematopoietic stem cells. It is mainly characterized by abnormal proliferation of primitive and immature myeloid cells in bone marrow and peripheral blood. The clinical manifestations are anemia, hemorrhage, infection and fever, visceral infiltration, abnormal metabolism, etc. Most cases are acute and severe, and the prognosis is dire.

[0003] In recent years, through the study of gene mutation frequency and some susceptibility biomarkers, it has been found that acute myeloid leukemia may be the result of a combination of genetics and environmental factors. Acute myeloid leukemia (AML) is a heterogeneous disease comprising many entities with distinct genetic abnormalities and clinical features.

[0004] Studying the g...

Claims

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