Plant nematode lateral line specimen preparation and observation method
A technique for specimen preparation and nematodes, applied in the field of biochemistry, can solve the problems of difficult to see lateral lines, distorted nematodes, and high operator skill requirements, and achieve the effects of long storage period, uniform coloring, and clear specimens
Inactive Publication Date: 2020-12-25
NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
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Problems solved by technology
However, the number of lateral lines is difficult to see under the microscope
The usual solution is to observe a large number of nematodes as much as possible, and search for some clear lateral lines to observe and take pictures. The second is to lightly press the temporary specimens of nematodes with fingers to distort the nematodes to better show the area where the lateral lines are located. The method requires high operator skills
Method used
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Embodiment Construction
[0017] The present invention will be further described according to the following examples, and the mode of the present invention includes but not limited to the following examples.
[0018] In this embodiment, the present invention includes the following steps:
[0019] a. Killing and immobilization of nematodes: transfer the nematode liquid into a 500 μL plastic tube, and centrifuge at 2000 r / min for 3 min to make the nematodes settle to the bottom of the plastic tube. Use a pipette to slowly suck out the water in the upper layer of the plastic tube, so that only a small amount of nematode liquid (about 10 μL) remains in the tube, and then add 95°C nematode fixative solution (40% formaldehyde: glycerol: distilled water = 10: 2:88), while killing and immobilizing nematodes (must be immobilized for more than 24h).
[0020] b. Centrifuge the plastic tube at 2000r / min for 3min to make the nematode sink at the bottom of the tube. After absorbing the upper layer of nematode fixat...
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Abstract
The invention discloses a plant nematode lateral line specimen preparation and observation method. The method comprises the following steps: adding a nematode fixing solution into residual nematode-containing liquid; further centrifuging the nematode stationary liquid, adding a glycerol aqueous solution, and standing at a constant temperature of 35-40 DEG C for 40-50 hours to complete dehydration;under an anatomical lens, selecting dehydrated adults, and performing segment cutting treatment on the nematodes, wherein the segment length is 5-10 [mu] m; transferring the cut nematode fragments into glycerol, and covering the cut nematode fragments with cover glass. The nematode lateral line preparation method is simple, convenient, safe, clear and complete in specimen, uniform in coloring, simple, convenient, long in storage life and suitable for plant nematode specimen side line observation.
Description
technical field [0001] The invention relates to the field of biochemistry, in particular to a method for preparing and observing plant nematode lateral line specimens. Background technique [0002] Plant nematodes belong to the nematode phylum in invertebrates, and can parasitize plant roots, stems, leaves, fruits, seeds and other parts. Currently, nematode identification methods used in the field of nematode classification and identification mainly include morphological identification methods and molecular biological identification methods . [0003] In the study of plant nematode detection and identification, the number of nematode lateral lines is one of the important characteristics of nematode species identification. However, the number of lateral lines is difficult to see under a microscope. The usual solution is to observe a large number of nematodes as much as possible, and search for some clear lateral lines to observe and take pictures. The second is to lightly p...
Claims
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IPC IPC(8): A01N1/00G01N21/84
CPCA01N1/00G01N21/84
Inventor 顾建锋方亦午刘乐乐吕晓玲
Owner NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE