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Method for establishing raspberry polyphenol fingerprint spectrum

A raspberry polyphenol and fingerprint technology is applied in the field of establishing the raspberry polyphenol fingerprint, which can solve the problems of large differences in polarity and structure, long time, poor separation effect, etc., and achieves high accuracy and method. Simple, reproducible results

Active Publication Date: 2020-12-25
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Description
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  • Application Information

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Problems solved by technology

[0003] There are many kinds of raspberry polyphenols, some compounds are similar in polarity and structure and difficult to separate and quantify, and some polarity and structure are quite different, and it takes a long time and more complicated conditions to separate
The previous high-performance liquid chromatography (HPLC) had a poor separation effect on this, and it was difficult to effectively separate the various components of raspberry polyphenols. However, ultra-high performance liquid chromatography (UPLC) increased the analytical throughput on the basis of HPLC. Quantity, sensitivity and chromatographic peak capacity, increasing the possibility of effective separation of components

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  • Method for establishing raspberry polyphenol fingerprint spectrum
  • Method for establishing raspberry polyphenol fingerprint spectrum
  • Method for establishing raspberry polyphenol fingerprint spectrum

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Embodiment Construction

[0024] The present invention will be further elaborated below in conjunction with the accompanying drawings and embodiments.

[0025] A method for establishing a raspberry polyphenol fingerprint, said method comprising the following steps:

[0026] Step 1. Component extraction: raspberry fruit, liquid nitrogen quick-cooling sample and use a homogenizer to pulverize and grind into powder, take 0.5 g of the sample to be tested, add 5 ml of 70% methanol aqueous solution, shake well, ultrasonicate at 60 degrees Celsius, and process for 50 minutes , centrifuged at 3000g for 10min, and the supernatant was filtered through a 0.45μm microporous membrane to obtain the sample extract to be tested.

[0027] Step 2. The sample extract prepared in step 1 was separated by ultra-high performance liquid chromatography (UPLC) analysis (WatersCorp., Milford, MA, USA, ACQUITY UPLC HSS T3 column (1.8×m, 2.1×150 mm), The chromatogram can be separated by UPLC.Test conditions: injection volume 5μL,...

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Abstract

The invention discloses a method for establishing a raspberry polyphenol fingerprint spectrum. A plurality of polyphenol substances are successfully separated under specific conditions based on ultra-high performance liquid chromatography (UPLC), and substance components are identified under specific conditions by utilizing tandem mass spectrometry. Common characteristic peaks of raspberries withhigh content of 8, stable retention time and different sources are selected as fingerprint spectrums, and the fingerprint spectrums have the advantages of being efficient in detection, high in resolution capability and capable of reflecting the difference between materials to the maximum extent, so that the fingerprint spectrums can be used for identification of counterfeit traditional Chinese medicinal materials and source analysis of the materials.

Description

technical field [0001] The invention relates to the field of chemical analysis and detection, in particular to a method for establishing a raspberry polyphenol fingerprint. Background technique [0002] Polyphenols are important health care or medicinal ingredients, widely distributed in fruits and Chinese herbal medicines, which have the functions of anti-oxidation, scavenging free radicals in the body, anti-inflammatory and antibacterial, and inhibiting malignant tumors. Many of the important components and contents are used as detection indicators for evaluating Chinese herbal medicines and health foods. [0003] There are many kinds of raspberry polyphenols, some compounds are similar in polarity and structure and difficult to separate and quantify, and some polarity and structure are quite different, requiring longer time and more complicated conditions to separate. The previous high-performance liquid chromatography (HPLC) had a poor separation effect on this, and it ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N30/8686G01N2030/146
Inventor 李小白孙健金亮任江剑
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES