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Method for improving stability of oyster respiratory rate

A technology of respiration rate and stability, applied in the field of improving the stability of oyster respiration rate, can solve problems such as low flux, and achieve the effects of improving stability, reducing fluctuation and improving efficiency

Active Publication Date: 2020-12-29
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the throughput of simultaneous measurement of shellfish respiration rate is low, and there is no related technical report on the monitoring method to improve the stability of oyster respiration rate, which poses a severe challenge to the high-throughput and accurate determination of oyster respiration rate.

Method used

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  • Method for improving stability of oyster respiratory rate
  • Method for improving stability of oyster respiratory rate
  • Method for improving stability of oyster respiratory rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Depend on figure 1 It can be seen that the device is a pretreatment device and a respiration rate measurement device, and the scattered light source is placed above the pretreatment device and the respiration rate measurement device; A light intensity sensor 4 is arranged, and a temperature control rod 2 is arranged on the inner wall of the temporary raising container 5 .

[0028] The respiration rate measuring device comprises a magnetic rotary stirrer 14 and a water bath temperature control tank 7 thereon, and at least one transparent breathing bottle 10 is placed in the water bath temperature control tank 7, which is connected to the dissolved oxygen measuring instrument 8 by wires, and the water bath temperature control Water inlet holes 6 and water outlet holes 11 are respectively provided on both sides of the tank 7 .

[0029] The bottom of each transparent breathing bottle 10 is provided with a magnetic rotator 13, and a grid frame 12 is provided inside, and an ...

Embodiment 2

[0034] In November 2019, in the shellfish holding room of the Institute of Oceanology, Chinese Academy of Sciences, the doors and windows of the room were shaded. Select oysters in good health, remove the attached organisms and impurities on the surface of the shell, and use about 5-10kg / m 3 Place the oyster to be tested with the left shell facing down on the mesh plate in a cylindrical transparent plastic holding container with a diameter of 60 cm filled with normal temperature seawater, set the air conditioner in the room to 23°C, Put in the temperature control rod (WK-3000, Zhongkehai) and set it to 23°C, and use the air pump to continuously inflate the water body during the 48h pretreatment period to keep the dissolved oxygen above 8mg / L, and use 2g / m 3 The standard is to feed microalgae dry powder once every 12 hours. An LED light source of the same type with the same power is set above the holding container and the test equipment respectively, and a light intensity sens...

Embodiment 3

[0041] In June 2020, in the living shellfish holding room of the Institute of Oceanology, Chinese Academy of Sciences, after the room was shaded. Select oysters in good health, remove the attached organisms and impurities on the surface of the shell, and pretreat this batch of oysters with the same equipment and conditions in Example 1. After 72 hours of pretreatment, randomly select 6 oysters after the treatment. Real-time monitoring of respiration rate values ​​at 8:30, 11:30, 15:30 and 19:00 in a day (measured at each time point for 1.5h) under light conditions (see Figure 3A ).

[0042] Randomly select 6 oysters in the same batch without pretreatment, and detect their oxygen consumption values ​​at 4 identical time points under the same equipment and the same light conditions in real time, as the control group of this embodiment (see Figure 3B ).

[0043] The living shellfish holding room mentioned above was not subjected to any shading treatment, and individuals of si...

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Abstract

The invention belongs to the field of shellfish physiological status monitoring, and particularly relates to a method for improving the stability of the oyster respiratory rate. Oysters are pretreatedin a pretreatment device under proper illumination intensity to tend to be stable, then the oysters are placed in a respiratory rate measuring device, so that the oysters are stirred under the properpretreatment illumination intensity and the test temperature of experiment design to be domesticated, then respiration of the oysters is kept in a stable state, and then the accurate and stable respiratory rate of the oysters is obtained. Compared with a traditional hydrostatic method measurement technology only controlling water temperature, by means of the method, fluctuation of the individualrespiratory rate of the oysters is remarkably reduced, and the stability of measurement of the oyster respiratory rate is improved. According to the method, fluctuation of the oyster respiratory ratecan be rapidly restrained in a high-flux and low-cost manner, the measurement precision of the oyster respiratory rate is remarkably improved, and important technical support can be provided for earlywarning and forecasting of oyster large-scale death, resistance breeding, breeding capacity evaluation and the like.

Description

technical field [0001] The invention belongs to the field of shellfish physiological state monitoring, in particular to a method for improving the stability of the respiration rate of oysters. Background technique [0002] Oysters are temperature-changing animals that naturally distribute in the mid-tidal zone to the subtidal zone in the intertidal zone, and their physiological state is highly susceptible to environmental changes. Respiration rate is an important indicator of oyster metabolism and physiological state, and is also an important parameter for constructing its physiological energy model. At present, the static water method is the mainstream method for measuring the oxygen consumption rate of oysters. In this method, there may be various environmental differences in the process of putting the oysters into the breathing bottle, which may lead to the stress response of the oysters, and then affect the accuracy of the oxygen consumption rate measurement. At present...

Claims

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Application Information

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IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 丛日浩赵泽民张渴新李莉张国范王威王鲁平
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI