Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of maize elicitor peptide gene zmpep1 in improving plant resistance to Verticillium wilt

An elicitor, Verticillium wilt technology, applied in the field of genetic engineering, to achieve the effect of improving resistance

Active Publication Date: 2022-08-05
SOUTHWEST UNIV
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether ZmPep1 can improve the disease resistance of other plants, especially the resistance to facultative trophic pathogens such as Verticillium wilt, has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of maize elicitor peptide gene zmpep1 in improving plant resistance to Verticillium wilt
  • Application of maize elicitor peptide gene zmpep1 in improving plant resistance to Verticillium wilt
  • Application of maize elicitor peptide gene zmpep1 in improving plant resistance to Verticillium wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Cloning of maize ZmPep1 gene

[0041] 1. Extraction of total RNA from maize genome and synthesis of cDNA

[0042] The 14-day-old maize seedlings were taken, and the base of the petiole was lightly clipped with tweezers to induce the expression of ZmPep1 gene. After 6 hours, the damaged leaves were snap-frozen in liquid nitrogen immediately, and ground into powder, then the total RNA of the leaves was extracted with Promoga's plant rapid RNA extraction kit, and cDNA was synthesized by TaKaRa's one-strand cDNA synthesis kit. RNA extraction and cDNA synthesis were carried out according to the kit instructions. The detailed operation process is as follows:

[0043] Take fresh corn leaves and freeze them in liquid nitrogen and grind them into powder. Take about 100 mg of the powder and put it into a 1.5 mL centrifuge tube without nuclease. Immediately add 500 μL of RNA lysis solution. Tissue, then add 300 μL of diluent, invert the centrifuge tube 3-4 times to m...

Embodiment 2

[0051] Example 2. Construction of plant expression vector constitutively expressing ZmPep1 gene and acquisition of engineered bacteria

[0052] The above-mentioned cloned vector engineering bacterial plasmids verified to be correct by sequencing were extracted, and double-enzymatically digested with BamHI and KpnI. The digested products were subjected to agarose gel electrophoresis to recover the target fragment ZmPep1. At the same time, the plant expression vector pLGN was digested with BamHI and KpnI. After the digestion was completed, agarose gel electrophoresis was performed to recover large fragments. The recovered gene and vector fragment were ligated by T4 DNA ligase, and the ligated product was transferred into Escherichia coli DH5α by heat shock method. The plasmid of the E. coli engineering bacteria was extracted, and then double-enzyme digestion was performed with BamHI and KpnI for verification. The transformed bacteria that obtained the ZmPep1 restriction fragment...

Embodiment 3

[0054] Example 3. Genetic transformation of Arabidopsis, screening of transgenic Arabidopsis and analysis of transcriptional expression levels

[0055] 1. Genetic transformation of Arabidopsis

[0056] Referring to Steven J.Clough and Andrew F.Bent's (1998) method of flower soaking transformation, the wild-type Arabidopsis thaliana was used as material for genetic transformation, and the seeds after Agrobacterium soaking were harvested after the seeds were mature.

[0057] 2. Screening of transgenic Arabidopsis plants

[0058] The seeds harvested after the genetic transformation by the flower dip transformation method were sterilized with 75% alcohol for 15 minutes, and then all inoculated on a screening plate with an additional 100 mg / L Km for germination. If the grown seedlings were green, they were transgenic plants. When the leaves are above, transplanted into the special soil for culturing Arabidopsis (grass carbon soil: vermiculite: perlite is 3: 1: 1), and the seedling...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of the maize elicitor peptide gene ZmPep1 in improving the resistance of plants to Verticillium wilt. By cloning the elicitor peptide gene ZmPep1 in maize, a molecular biology method is used to construct a plant expression vector for constitutive expression of ZmPep1, Then, using genetic engineering method, ZmPep1 gene was introduced into plants, and transgenic Arabidopsis thaliana, tobacco and cotton lines with normal transcription and expression were obtained; The ZmPep1 gene can be used to improve the resistance of plants to Verticillium wilt, which is of great significance for plant disease resistance genetic engineering.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the application of the maize elicitor peptide gene ZmPep1 in improving the resistance of plants to Verticillium wilt, and also relates to the use of constitutively expressing the maize elicitor peptide ZmPep1 gene to improve the resistance of plants to Verticillium wilt. method. Background technique [0002] Plant diseases are one of the long-term natural disasters that endanger agricultural production, with an annual loss of more than 10% globally due to diseases (Feng Zhanshan et al., 2013). Plant diseases not only cause crop yield reduction, but also seriously threaten the quality and safety of agricultural products and international trade, and even cause food shortages and a series of social problems in severe cases (Punja, 2004). The harm of pathogenic bacteria not only causes direct economic losses, but also produces toxins, which brings serious food safety problems. Th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H5/00A01H6/20A01H6/82A01H6/60
CPCC12N15/8282
Inventor 李先碧于晓涵郑雪丽李美华林道尧唐梦裴炎金丹范艳华侯磊赵娟
Owner SOUTHWEST UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com