Blood retinal outer barrier model, construction method thereof and culture medium combination adopted for constructing model

A construction method and medium technology, applied in the field of medium combination, blood-retinal barrier model and its construction, can solve the problems of inability to accurately construct oBRB model derived from patients
CN112175907AActive Publication Date: 2021-01-05AIER EYE HOSPITAL GRP CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
AIER EYE HOSPITAL GRP CO LTD
Publication Date
2021-01-05

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Abstract

The invention relates to the field of biological medicine, in particular to a blood retinal outer barrier model, a construction method thereof and a culture medium combination adopted for constructingthe model. The invention provides a construction method of a scaffold-free material integrated oBRB model differentiated from human iPSC. The preparation method comprises the following main steps offirstly, inducing embryoid mesoderm cell spheres from iPSC in a 3D culture manner, then embedding the cell spheres into collagen I, and inducing the collagen I to form a vascular network sheet. And inoculating the RPE cells from the iPSC to the vascular network sheet, and co-culturing for a period of time to form an integral structure. The iPSC differentiation technology and the tissue engineeringtechnology are combined, the blood-retinal outer barrier structure is better simulated, a new research way for obtaining the oBRB model is provided, and a new replacement way is provided for stem cell treatment of AMD and other diseases.
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Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to a blood-retinal outer barrier model and a construction method thereof, and a culture medium combination used to construct the model. Background technique

[0002] The blood-retinal barrier (BRB), consists of an inner barrier and an outer barrier. The retinal capillary endothelium and its connections form the inner blood retinal barrier (iBRB); the choriocapillary layer and retinal pigment epithelium (RPE) form the outer blood retinal barrier (oBRB). Its main function is through the formation of tight junctions between RPE cells. The RPE is a monolayer of hexagonal cells with folds in the base membrane for transport and constitutes the inner layer of Bruch's membrane. Part of Bruch's membrane is composed of RPE and the other part is synthesized by choroidal cells. In addition to the RPE as a selective permeability barrier to the retina, Bruch's membrane also forms a barrier to certai...

Claims

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