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Use of hsa_circ_0069922 as marker

A marker and drug technology, applied in the field of clinical screening of SAI, biomedicine and molecular biology, can solve the problems of aggravating stroke injury and increasing expression, achieving good sensitivity and specificity, and high diagnostic value.

Active Publication Date: 2021-01-05
NANJING FIRST HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, circHECTD1 can compete with miR-142, leading to an increase in the expression of the downstream target gene TIPARP of miR-142, causing the activation of astrocytes and aggravating stroke damage.

Method used

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  • Use of hsa_circ_0069922 as marker
  • Use of hsa_circ_0069922 as marker
  • Use of hsa_circ_0069922 as marker

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Experimental program
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Embodiment

[0024] The genetic research group of the present invention screened by SAI in the early stage, selected 5 pairs of PBMCs of SAI and non-infected patients for high-throughput sequencing, and screened out circRNA molecules with high expression and good difference for screening verification analysis. The present invention selects hsa_circ_0069922 to be used for SAI screening through subsequent verification and statistical analysis.

[0025] Study Objects and Samples

[0026] A total of 60 cases of SAI and non-infected patients admitted to Nanjing First Hospital from December 2018 to March 2020 were selected. Among them, 30 cases of SAI and 30 cases of non-infected patients were confirmed by imaging examination. Use EDTA anticoagulant tubes to obtain 3ml-5ml of human peripheral blood, take appropriate methods to preserve and process the samples within 2 hours (to avoid RNA degradation).

[0027] Isolation of PBMCs

[0028] Take peripheral blood and transfer it to a 15ml centrifu...

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Abstract

The present invention discloses a use of hsa_circ_0069922 as a marker in preparing a product for screening stroke-associated infection (SAI). The product for screening the stroke-related infection comprises a reagent or a drug for detecting an expression level of the hsa_circ_0069922 in peripheral blood mononuclear cells of a patient. The present invention develops the new molecular marker hsa_circ_0069922 for screening diagnosis of the SAI and a new reference for screening the SAI. The marker hsa_circ_0069922 is high in diagnostic value, a ROC curve analysis shows that patients with the SAI can be well distinguished and screened, an area under the curve is 0.832, and the marker hsa_circ_0069922 has good sensitivity and specificity and is expected to become an important index applied to evaluation of clinical diagnosis and treatment of the SAI.

Description

technical field [0001] The invention relates to the application of hsa_circ_0069922 as a marker in the preparation of products or medicines for stroke-related infection screening, belongs to the technical field of biomedicine and molecular biology, and specifically relates to the field of clinical screening of SAI. Background technique [0002] As a global public health problem, stroke can not only cause permanent disability, but also is the second leading cause of human death. In the past 30 years, the incidence of stroke in my country has risen sharply, and it has become the number one cause of death among Chinese citizens. Stroke includes ischemic stroke and hemorrhagic stroke, and ischemic stroke accounts for about 85% of all strokes. Although the treatment of ischemic stroke has developed rapidly in recent years, there are still some unresolved clinical problems in the treatment of ischemic stroke, among which stroke-associated infection (SAI) has attracted worldwide a...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11A61K45/00A61P31/00A61P9/10A61P25/00
CPCC12Q1/6883A61K45/00A61P31/00A61P9/10A61P25/00C12Q2600/118C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 邓齐文周俊山施洪超孙慧玲李烁
Owner NANJING FIRST HOSPITAL
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