Multiplex PCR detection kit for multiple pathogenic bacteria of waterfowls and application multiplex PCR detection kit

Abstract

The invention relates to the technical field of biological detection, in particular to a multiplex PCR detection kit for multiple pathogenic bacteria of waterfowls and application of the multiplex PCRdetection kit. The kit comprises riemerella anatipestifer, escherichia coli, pasteurella and staphylococcus aureus DNA extracting solutions, riemerella anatipestifer ompA-F/R primers, pasteurella kmt-F/R primers, escherichia coli phoA-F/R primers, staphylococcus aureus invA-F/R primers, a 10 * PCR reaction buffer solution, a Pfu PCR mix, and ddH2O. According to the multiplex PCR detection kit formultiple pathogenic bacteria of the waterfowls and the application of the multiplex PCR detection kit, a multiplex PCR detection method capable of simultaneously detecting four waterfowl-derived mainpathogenic bacteria comprising the riemerella anatipestifer, the escherichia coli, pasteurella and staphylococcus aureus is established, the multiplex PCR detection method has the characteristics ofeconomy, rapidness, high efficiency, strong specificity and good repeatability, and can be used for rapidly detecting the waterfowl-derived main pathogenic bacteria.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a multiple PCR detection kit for various pathogenic bacteria in waterfowl and an application thereof. Background technique [0002] my country is a big country for breeding and consuming waterfowl, and the breeding volume accounts for more than 80% of the world's total. Due to the living habits of grazing and swimming in the breeding, and the influence of factors such as environmental control and breeding level, it leads to a high incidence of bacterial diseases. Serious threat to the development of waterfowl industry. [0003] R. anatipestifer, Escherichia coli, Pasteurella, and Staphylococcus are currently the pathogenic bacteria with the highest detection rate of bacterial diseases in waterfowl, with extremely high morbidity and fatality rates, all of which can cause ataxia, nervous symptoms. R. anatipestifer and Escherichia coli can cause fibrous pericarditis an...

AI Technical Summary

Problems solved by technology

Epidemiological surveys of waterfowl in recent years have shown that mixed infections and secondary infections of various pathogens are common, so it is difficult to quickly determine the cause by clinical autopsy

[0004] In terms of bacterial isolation and identification, R. anatipestifer, Pasteurella, Escherichia coli and Staphylococcus can grow on TSA medium containing serum, and the colonies are similar in shape and difficult to distinguish. However, duck plague cannot be quickly distinguished by Gram staining. Ribella, Pasteurella, and Escherichia coli (the above three bacteria are Gram-negative bacteria), the traditional biochemical test cycle is longer

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