Optical characteristic change detection system and method in action potential transmission process in nerves

Abstract

The embodiment of the invention provides an optical characteristic change detection system and method in a nerve action potential transmission process, and the system comprises an optical electrical preparation module which provides electrical stimulation for a stimulated point of a nerve sample, generates an action potential signal, outputs a frequency-tunable mid-far infrared light signal, and realizes the synchronization and time delay control of middle and far infrared light signals and action potential signals; a stimulation transmission module which comprises a confocal positioning lightpath of a visible light band and a confocal light path of a middle and far infrared band; and an optical signal detection module which receives the mid-far infrared light signal output by the stimulation transmission module and performs characteristic detection to obtain an action potential signal envelope. According to the system and the method provided by the embodiment of the invention, the action potential is generated in the nerve through electrical stimulation, the optical characteristic change detection in the nerve action potential transmission process is realized based on the synchronous mid-far infrared laser, the high-time resolution detection of the biological characteristics in the nerve action potential transmission process can be realized, and the detection precision is improved.

Description

technical field [0001] The invention relates to the technical field of biophotonics, in particular to a system and method for detecting changes in optical characteristics during the transmission of action potentials in nerves. Background technique [0002] Nerve action potential transmission can be explained by the Hodgkin-Huxley model (H-H model). In 1963, the author of this model won the Nobel Prize, and the H-H model has also become the basis of modern neuroscience. The action potential in the nerve can be measured by the patch clamp technique, and the single or multiple current sensitivity of 1pA, the spatial resolution of 1um and the time resolution of 10us can be measured near the cell membrane by using the drawn micron-scale microelectrode tip. An ion channel current signal. Based on this current signal, the electrical properties of the nerve can be experimentally characterized. The H-H model believes that the protein molecules in the cell membrane play the role of ...

AI Technical Summary

Problems solved by technology

However, how to study the change of optical properties during action potential transmission from the perspective of combining physics and electricity has not been reported yet.

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