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A nucleic acid extraction and purification device

A purification device and nucleic acid technology, applied in the field of nucleic acid purification, can solve problems such as low purity, long purification time, and low efficiency

Active Publication Date: 2021-08-24
上海钧济医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing nucleic acid extraction and purification devices can only perform single-stage purification, with long purification time, low efficiency and relatively low purity

Method used

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  • A nucleic acid extraction and purification device
  • A nucleic acid extraction and purification device
  • A nucleic acid extraction and purification device

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] combine Figure 1-6 , a nucleic acid extraction and purification device, comprising a feed pipe 1, a primary purification mechanism 2, a reagent feed mechanism 7, a transfer mechanism 4, a secondary purification mechanism 6 and a sample discharge pipe 8, and the feed pipe 1 is connected to a The left side of the primary purification mechanism 2, the right side of the primary purification mechanism 2 is connected to the left side of the transfer mechanism 4 through the first connecting pipe 3, the upper end of the primary purification mechanism 2 is connected to a reagent feeding mechanism 7, the transfer The right side of the mechanism 4 is connected to the upper end of the secondary purification mechanism 6 through the second connecting pipe 5, the lower end of the secondary purification mechanism 6 is connected to the sample outlet pipe 8, and the first one-way valve 9 is installed in the first connecting pipe 3 , the second one-way valve 10 is installed in the second...

Embodiment 2

[0039] combine figure 1 with Figure 5 , a nucleic acid extraction and purification device, this embodiment further defines the present invention on the basis of embodiment 1.

[0040] A temperature sensor 14 is installed on the inner top wall of the second tank body 61, a heating plate 11 is installed on the inner side wall of the second tank body 61, and a controller 12 is installed on the outer side wall of the second tank body 61. , the controller 12 is electrically connected to the heating plate 11 and the temperature sensor 14 respectively.

[0041] Specifically, the temperature in the second tank body 61 is detected by the temperature sensor 14, and when the temperature in the second tank body 61 is not within the rated range set by the controller 12, the controller 12 controls the heating plate 11 to perform the primary purification. The protein is heated to avoid the decomposition of the precipitated protein and affect the purity of the extracted nucleic acid.

[0...

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Abstract

The invention discloses a nucleic acid extraction and purification device, which comprises a feeding pipe, a primary purification mechanism, a reagent feeding mechanism, a transfer mechanism, a secondary purification mechanism and a sample outlet tube, and the feeding pipe is connected to the primary purification mechanism. On the left side, the right side of the primary purification mechanism is connected to the left side of the transfer mechanism through the first connecting pipe, the upper end of the primary purification mechanism is connected to a reagent feeding mechanism, and the right side of the transfer mechanism is connected to the second The upper end of the first-stage purification mechanism is connected, the lower end of the second-stage purification mechanism is connected with a sample outlet pipe, a first one-way valve is installed in the first connecting pipe, and a second one-way valve is installed in the second connecting pipe. The present invention realizes the multi-stage extraction and purification of materials through the mutual cooperation of the primary purification mechanism, the reagent feeding mechanism, the transfer mechanism and the secondary purification mechanism, and improves the extraction and purification efficiency and purity.

Description

technical field [0001] The invention relates to the technical field of nucleic acid purification, in particular to a nucleic acid extraction and purification device. Background technique [0002] Nucleic acid is a biomacromolecular compound synthesized by many nucleotides. It is one of the most basic substances of life. Nucleic acid widely exists in all animal and plant cells and microorganisms. Nucleic acid in organisms is often combined with protein to form nucleoprotein. Different Nucleic acid has different chemical composition and nucleotide arrangement sequence. According to different chemical composition, nucleic acid can be divided into ribonucleic acid and deoxyribonucleic acid. The extraction methods of general equipment for nucleic acid are: DNA extraction and purification method and RNA extraction and purification method. Existing nucleic acid extraction and purification devices can only perform single-stage purification, with long purification time, low efficienc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/02C12M1/12C12M1/38C12M1/36C12M1/34C12M1/00C07K1/14C07K1/36C07K1/30
CPCC07K1/14C07K1/30C07K1/36C12N15/1003
Inventor 陈小翠
Owner 上海钧济医学检验所有限公司
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