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A kind of non-viral nanoparticle for gene molecular delivery and its preparation method and application

A nanoparticle, non-viral technology, applied in gene therapy, nanotechnology, nanotechnology, etc., can solve problems such as limited application and low gene transfection efficiency, and achieve high-efficiency transfection, good biological safety, and mild reaction conditions Effect

Active Publication Date: 2022-04-08
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these biocompatible polycations have many attractive properties, low gene transfection efficiency still limits their application as gene carriers

Method used

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  • A kind of non-viral nanoparticle for gene molecular delivery and its preparation method and application
  • A kind of non-viral nanoparticle for gene molecular delivery and its preparation method and application
  • A kind of non-viral nanoparticle for gene molecular delivery and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Follow the steps below to prepare non-viral nanoparticles for gene molecular delivery:

[0033] At room temperature, add 1 g of dextran into 40 mL of dimethyl sulfoxide, stir until completely dissolved to obtain a dextran solution, add 2 g of 1,1'-carbonyldiimidazole into the dextran solution and stir for 12 hours, then add 4 mL of bis-(3-di Methylpropylamino) amine, stirring and reacting for 24 hours to obtain a reaction solution, centrifuging the reaction solution to obtain a precipitate, repeatedly washing the precipitate with deionized water to remove unreacted dextran to obtain non-viral nanoparticles for gene molecule delivery.

[0034] The application of the non-viral nanoparticles prepared by the above steps in the delivery of gene molecules comprises the following steps:

[0035] In a sterile environment, add 0.1 g of non-viral nanoparticles into 10 mL of PBS, stir until completely dispersed to obtain a non-viral nano-particle solution, add 2 μg of the target g...

Embodiment 2

[0037] Follow the steps below to prepare non-viral nanoparticles for gene molecular delivery:

[0038] At room temperature, add 1 g of dextran into 40 mL of dimethyl sulfoxide, stir until completely dissolved to obtain a dextran solution, add 2 g of 1,1'-carbonyldiimidazole into the dextran solution and stir for 24 hours, then add 6 mL of bis-(3-di Methylpropylamino) amine, stirring and reacting for 24 hours to obtain a reaction solution, centrifuging the reaction solution to obtain a precipitate, repeatedly washing the precipitate with deionized water to remove unreacted dextran to obtain non-viral nanoparticles for gene molecule delivery.

[0039] The application of the non-viral nanoparticles prepared by the above steps in the delivery of gene molecules comprises the following steps:

[0040]In a sterile environment, add 0.1 g of non-viral nanoparticles into 10 mL of PBS, stir until completely dispersed to obtain a non-viral nano-particle solution, add 4 μg of the target ge...

Embodiment 3

[0042] Follow the steps below to prepare non-viral nanoparticles for gene molecular delivery:

[0043] At room temperature, add 1 g of dextran into 40 mL of dimethyl sulfoxide, stir until completely dissolved to obtain a dextran solution, add 2 g of 1,1'-carbonyldiimidazole into the dextran solution and stir for activation for 24 hours, then add 8 mL of bis-(3-di Methylpropylamino) amine, stirring and reacting for 24 hours to obtain a reaction solution, centrifuging the reaction solution to obtain a precipitate, repeatedly washing the precipitate with deionized water to remove unreacted dextran to obtain non-viral nanoparticles for gene molecule delivery.

[0044] The application of the non-viral nanoparticles prepared by the above steps in the delivery of gene molecules comprises the following steps:

[0045] In a sterile environment, add 0.1 g of non-viral nanoparticles into 10 mL of PBS, stir until completely dispersed to obtain a non-viral nano-particle solution, add 6 μg ...

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Abstract

The invention provides a non-viral nanoparticle for gene molecular delivery, which is prepared by the following steps: add dextran into dimethyl sulfoxide at room temperature, stir until completely dissolved to obtain a dextran solution, and mix 1,1'-carbonyl di Imidazole was added into the dextran solution and stirred for 12-24h, then bis-(3-dimethylpropylamino)amine was added dropwise, stirred for 24-36h to obtain a reaction solution, the reaction solution was centrifuged to obtain a precipitate, and deionized water The precipitate is washed repeatedly to remove unreacted dextran to obtain non-viral nanoparticles for gene molecule delivery. The invention also discloses a preparation method of the non-virus nano particle. The non-viral nanoparticles provided by the invention can not only avoid the strong immunogenicity and potential infectivity of virus transfection, but also improve the transfection efficiency of the polycationic polymer.

Description

technical field [0001] The invention relates to a non-viral nano particle, in particular to a non-viral nano particle used for gene molecule delivery and its preparation method and application. Background technique [0002] Genetic material as a therapeutic agent offers many potential benefits in the treatment of a variety of diseases. In gene therapy, genes are delivered into target host cells to produce or silence functional proteins for the purpose of curing diseases. Normally, negatively charged oligonucleotides cannot penetrate the plasma membrane without a delivery system, thus, highly efficient gene delivery systems called "gene vectors" were created. Compared with highly immunogenic and potentially infectious viral vectors, nonviral vectors have been shown to have the advantage of attenuating unwanted clinical responses and enabling controlled gene delivery. Among them, by utilizing the charge-charge interaction with polyanionic nucleic acids, cationic polymer mate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K9/14B82Y5/00
CPCA61K48/0041A61K9/14B82Y5/00
Inventor 高明郑立丁燕锋冯娴婧胡文文李志敏严国华
Owner GUANGXI MEDICAL UNIVERSITY
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