Preparation method of the reference substance of cyanidin-3-coumaroyl-diglucose-5-glucoside

Abstract

The present invention provides a method for preparing a reference substance of cyanidin-3-coumaryl-diglucose-5-glucoside with a purity of more than 98%, comprising enrichment and concentration process, chromatographic column purification process and solidification process, The solution of the target substance obtained in the chromatographic column preparation process is concentrated to a concentration of cyanidin-3-coumaroyl-diglucose-5-glucoside of 5% to 30%, and the concentrated solution is freeze-dried to obtain cyanidin The solid powder of ‑3‑coumaroyl‑diglucose‑5‑glucoside, the stability of cyanidin‑3‑coumaroyl‑diglucose‑5‑glucoside was verified, and it was found that its formate could exist stably , It is also found that the current anthocyanin standard substance has obtained a solid powder reference substance, which is very beneficial to the storage and distribution of the reference substance.

Description

technical field

[0001] The present invention relates to a preparation method of cyanidin-3-coumaroyl-diglucose-5-glucoside, in particular to high-purity cyanidin-3-coumaroyl-diglucose-5-glucoside from purple cabbage Preparation method of solid reference substance of glucoside. Background technique

[0002] cyanidin-3-coumaroyl-diglucose-5-glucoside is a kind of anthocyanin, the structure is as shown in the following formula 1, it was first reported structure by Ole-P. Johansen et al. (Phytochemistry, 1991, 30 , 4137-4141).

[0003] Formula 1

[0004] The researchers also found it in purple cabbage. Purple cabbage is a common vegetable, also known as purple cabbage, red cabbage, purple cabbage, and red cabbage. Varieties, which originated in the Mediterranean Sea and western and southern Europe, are currently grown and eaten all over the world, and have always been a research hotspot for medicinal and edible homologous products. In recent years, there have been many re...

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