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Enzyme preparation microbial strain liquid fermentation device and fermentation process using same

A technology of microbial strains and liquid fermentation, which is applied in enzymology/microbiology devices, biochemical cleaning devices, bioreactor/fermentation tank combinations, etc., which can solve the problem of uneven distribution of dissolved oxygen, affecting fermentation effect, and insufficient stirring and other problems to achieve the effect of improving the mixing effect, increasing the degree of mixed flow, and reducing the access

Active Publication Date: 2021-01-22
天津正鑫浩司科技有限公司
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Problems solved by technology

[0004] In view of this, the present invention aims to propose a liquid fermentation device for enzyme preparation microbial strains and its fermentation process, to solve the problems of insufficient stirring and uneven distribution of dissolved oxygen in the fermentation process that affect the fermentation effect, and without affecting the dissolution Defoaming in the presence of oxygen

Method used

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  • Enzyme preparation microbial strain liquid fermentation device and fermentation process using same
  • Enzyme preparation microbial strain liquid fermentation device and fermentation process using same
  • Enzyme preparation microbial strain liquid fermentation device and fermentation process using same

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Embodiment Construction

[0048] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0049] The present invention will be described in detail below in conjunction with examples.

[0050] Such as Figure 1-4 Shown, a kind of enzyme preparation microbial strain liquid fermentation device comprises fermentor 1 and is arranged on the stirring mechanism inside fermentor 1; There is a feed port 22, and the bottom of the fermenter 1 is provided with a discharge port 23; specifically, the feed port 22 can be set below the liquid level line to facilitate feeding;

[0051] The liquid level line above the inside of the fermenter 1 is fixed with a horizontal screen 8, the top of the horizontal screen 8 is a defoaming zone, and the bottom of the horizontal screen 8 is a stirring zone;

[0052] The stirring mechanism includes a rotating shaft 2, a stirring blade 3, a stirring rod 4, a scraper 5 and a mo...

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Abstract

The invention provides an enzyme preparation microbial strain liquid fermentation device and a fermentation process using the same. A horizontal screen is fixedly arranged at a liquid level line abovethe interior of a fermentation tank, a defoaming area is arranged above the horizontal screen, and a stirring area is arranged below the horizontal screen; a vertical screen is fixedly connected to the side wall of a rotating shaft located in the upper defoaming area, and the lower end of the vertical screen is attached to the upper surface of the horizontal screen; a cooling coil sleeves the periphery of the rotating shaft in the lower stirring area, and the water inlet end and the water outlet end of the cooling coil penetrate out of the wall of the fermentation tank; and stirring blades and stirring rods are fixedly connected to the rotating shaft, and the scraping plates are attached to the inner side wall of the fermentation tank. The fermentation process performed by the device solves the problem that the fermentation effect is influenced due to insufficient stirring and non-uniform dissolved oxygen distribution in the fermentation process, and besides, defoaming can be performed under the condition of being free from influencing dissolved oxygen.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to an enzyme preparation microbial strain liquid fermentation device and a fermentation process thereof. Background technique [0002] There are two methods of enzyme fermentation production, one is solid fermentation, and the other is liquid submerged fermentation. The solid fermentation method is mainly used for commercial enzyme production from fungal sources. Among them, the production of amylase by Aspergillus oryzae and the production of protease by Aspergillus and Mucor have a long history in China and Japan. Although this culture method is simple, the operating conditions are not easy to control. Most enzymes are now produced by submerged cultures. [0003] Liquid submerged fermentation is also called submerged culture, which uses liquid medium, a stirring and aeration culture method carried out in a fermenter. During the fermentation process, the strains ...

Claims

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Application Information

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IPC IPC(8): C12M1/21C12M1/12C12M1/04C12M1/02C12M1/00B08B3/10B08B3/08
CPCB08B3/08B08B3/102C12M21/14C12M27/08C12M29/04C12M29/06C12M29/14C12M29/18C12M33/00C12M37/00C12M39/00C12M41/02C12M41/24
Inventor 卞亦冰
Owner 天津正鑫浩司科技有限公司
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