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Independent multichannel immunofluorescence microfluidic chip and immunofluorescence detection method

A microfluidic chip and immunofluorescence technology, applied in the direction of fluorescence/phosphorescence, measuring devices, instruments, etc., can solve the problems of unstable detection results, signal crosstalk, and limit the application range of chips, so as to improve detection accuracy and efficiency, reduce cost effect

Pending Publication Date: 2021-02-02
苏州含光微纳科技有限公司
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Problems solved by technology

[0005] However, the existing immune microfluidic chips all use a single-channel liquid-feeding reaction, and there is a problem of signal crosstalk when multiple items are tested, which limits the application range of the chip.
The existing multi-channel multi-target blood immunofluorescence chip adopts the method of equally dividing the sample liquid pool through the fluorescent antibody-coated area and the time-space valve, which fails to solve the detection results caused by various fluorescent antibodies in the coated area. unstable problem

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  • Independent multichannel immunofluorescence microfluidic chip and immunofluorescence detection method
  • Independent multichannel immunofluorescence microfluidic chip and immunofluorescence detection method
  • Independent multichannel immunofluorescence microfluidic chip and immunofluorescence detection method

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Embodiment Construction

[0028] The present invention will be described in further detail below through specific embodiments and accompanying drawings.

[0029] figure 1 It is a schematic diagram of the structure of the independent multi-channel immunofluorescence microfluidic chip of this embodiment, which contains three independent channels. In other embodiments, there may also be two or more than three independent channels, that is, the number of channels is at least two.

[0030] figure 1 In , the liquid inlet 1 is used to put the sample. Equal distribution channel 2 is used to realize precise liquid distribution. The purpose of using multiple curved pipes is to make the flow resistance of each channel consistent. The filter area 3 is used for filtering impurities in the sample. The buffer zone 4 is used to realize the buffering of the liquid, so that the liquid goes hand in hand. The fluorescent coating area 5 is used for the immobilization of fluorescent reagents. The time control valve 6...

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Abstract

The invention relates to an independent multichannel immunofluorescence microfluidic chip and an immunofluorescence detection method. The independent multi-channel immunofluorescence microfluidic chipcomprises at least two independent channels, each channel comprises an equivalent uniform flow channel, a filtering area, a buffer area, a fluorescence coating area, a time control valve, a detectionarea and a waste liquid area which are connected in sequence, and the equivalent uniform flow channels of all the channels are connected with the same liquid inlet. According to the independent multi-channel immunofluorescence microfluidic chip, liquid is equally divided in the sample feeding stage, different channels have independent fluorescence antibody coating areas, space-time valves and independent waste liquid areas, and the problem that different detection items may cause mutual interference is solved; each independent channel can integrate a plurality of detection items which do notinterfere with one another, so that the detection precision and efficiency are improved and the cost is reduced while high flux is realized.

Description

technical field [0001] The invention relates to the technical field of on-site rapid inspection, in particular to an independent multi-channel immunofluorescence microfluidic chip and an immunofluorescent detection method using the microfluidic chip. Background technique [0002] On-site rapid test (Point-of-Care Test, POCT), also known as point-of-care test, internationally known as POCT, is the fastest growing field in the in vitro diagnostic industry. [0003] In the existing POCT, immunofluorescence staining technique is a common method for observing the distribution and localization of proteins in cells. The traditional method of immunofluorescence staining needs to consume a lot of reagents, manpower and time, especially the consumption of some expensive antibodies, which limits the use of traditional methods. [0004] At present, chips using immunofluorescence detection technology include immunochromatography and fluorescent immunological microfluidic technology. Im...

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Application Information

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IPC IPC(8): G01N21/64G01N33/533
CPCG01N21/64G01N33/533
Inventor 宋璐吴烨娴朱玲英岳婕陈兢
Owner 苏州含光微纳科技有限公司
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