The invention discloses a
gene chip for integrated detection and an on-substrate amplification method. The on-substrate amplification method comprises the following steps: (1) fixing specific
nucleic acid primers on the substrate; (2) placing the substrate, on which the specific
nucleic acid primers are fixed, in an amplification tube; (3) adding prepared a
gene extract to be detected and an amplification
system to the amplification tube; (4) placing the amplification tube in a
gene amplification instrument for amplification; (5) after completion of the gene amplification, detecting fluorescent signals on the substrate so as to acquire genetic information. In the on-substrate amplification method, the general step of
nucleic acid hybridization is removed, the benefits are greatly increased, and the cost is reduced; the specific nucleic acid primers are fixed on the substrate, and then the specific nucleic acid primers and the substrate are placed in the amplification tube for on-substrate PCRs (
Polymerase Chain Reactions), so that the PCR reactions can be realized separately and the problem that primers interfere with one another during multiple PCRs can be solved; multiple pieces of genetic information can be detected integrally at the same time, and
contamination, which can be generated in the amplification process and the product transfer process, is avoided; the used instrument is simple and universal, so that a common PCR instrument and a
fluorescence microscope can complete the detection.